刘春丽,陈汉峰,杨 静,卢迪勋,黎俊威,吴邵敏,候少平.高效液相色谱法同时测定固体饮料中没食子酸、芦丁、鞣花酸和槲皮素的含量[J].食品安全质量检测学报,2024,15(24):63-70
高效液相色谱法同时测定固体饮料中没食子酸、芦丁、鞣花酸和槲皮素的含量
Simultaneous determination of gallic acid, rutin, ellagic acid and quercetin content in solid drinks by high performance liquid chromatography
投稿时间:2024-10-28  修订日期:2024-12-13
DOI:
中文关键词:  高效液相色谱法(HPLC)  没食子酸  芦丁  鞣花酸  槲皮素  食品
英文关键词:high performance liquid chromatography  gallic acid  rutin  ellagic acid  quercetin  solid drinks
基金项目:
作者单位
刘春丽 1.捷通(广州)检测技术服务有限公司 
陈汉峰 1.捷通(广州)检测技术服务有限公司 
杨 静 1.捷通(广州)检测技术服务有限公司 
卢迪勋 1.捷通(广州)检测技术服务有限公司 
黎俊威 1.捷通(广州)检测技术服务有限公司 
吴邵敏 1.捷通(广州)检测技术服务有限公司 
候少平 1.捷通(广州)检测技术服务有限公司 
AuthorInstitution
LIU Chun-Li 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
CHEN Han-Feng 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
YANG Jing 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
LU Di-Xun 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
LI Jun-Wei 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
WU Shao-Min 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
HOU Shao-Ping 1.Access (Guangzhou) Testing Technology Service Co., Ltd. 
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中文摘要:
      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时测定固体饮料中没食子酸、芦丁、鞣花酸和槲皮素含量的方法。方法 样品经15%二甲亚砜(dimethyl sulfoxide, DMSO)甲醇溶液萃取, 离心后取上清液, 经0.45 μm微孔滤膜过滤后用高效液相色谱仪分析。选用色谱柱Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm); 流动相A为乙腈, 流动相B为0.2%磷酸水溶液, 梯度洗脱; 流速为1.0 mL/min; 柱温为25 ℃; 进样体积 5 μL; 检测波长没食子酸为273 nm, 芦丁、鞣花酸和槲皮素为254 nm, 运行时间为55 min。结果 没食子酸、芦丁、鞣花酸和槲皮素分别在0.00072~0.02150 mg/mL (r2=0.9953)、0.00380~0.15000 mg/mL (r2=0.9998)、0.00033~0.01300 mg/mL (r2=0.9995)、0.00062~0.02500 mg/mL (r2=0.9999)范围内线性关系良好; 没食子酸、芦丁、鞣花酸、槲皮素的加标回收率在95.7%~109.7%之间, 相对标准偏差在0.7%~2.2%之间。本方法中检出限和定量限: 没食子酸分别为11.0 μg/g和33.0 μg/g; 芦丁分别为6.6 μg/g和20.0 μg/g; 鞣花酸分别为1.5 μg/g和5.2 μg/g; 槲皮素分别为3.5 μg/g和12.0 μg/g。结论 本方法前处理方法简单, 检测灵敏度高, 稳定性好, 并且可以有效地把食品样品中没食子酸、芦丁、鞣花酸和槲皮素几种植物化学物质同时准确检测, 可以作为成分复杂的固体饮料中多个植物化学物的质量控制的参考。
英文摘要:
      Objective To establish a method for the simultaneous determination of gallic acid, rutin, ellagic acid, and quercetin in solid drinks by high performance liquid chromatography (HPLC). Methods The sample was extracted with a 15% dimethyl sulfoxide (DMSO) methanol solution, centrifuged, and the supernatant was collected. The supernatant was filtered through a 0.45 μm microporous membrane and analyzed using HPLC. Select Agilent ZORBAX SB-C18 chromatographic column (4.6 mm×250 mm, 5 μm) was selected. Mobile phase A was acetonitrile, mobile phase B was 0.2% phosphoric acid aqueous solution, using gradient elution. The flow rate was 1.0 mL/min. The column temperature was at 25 ℃. Injection volume was 5 μL. The detection wavelengths for gallic acid was 273 nm, rutin, ellagic acid and quercetin were 254 nm, and the running time was 55 minutes. Results Gallic acid, rutin, ellagic acid, and quercetin showed good linear relationships within the ranges of 0.00072–0.02150 mg/mL (r2=0.9953), 0.00380–0.15000 mg/mL (r2=0.9998), 0.00033–0.01300 mg/mL (r2=0.9995), and 0.00062–0.02500 mg/mL (r2=0.9999), respectively. The spiked recovery rates of gallic acid, rutin, ellagic acid, and quercetin ranged from 95.7% to 109.7%, with relative standard deviations between 0.7% and 2.2%. The limit of detection of gallic acid in this method was 11.0 μg/g, and the limit of quantification was 33.0 μg/g. The limit of detection of rutin was 6.6 μg/g, and the limit of quantification was 20.0 μg/g. The limit of detection of ellagic acid was 1.5 μg/g, and the limit of quantification was 5.2 μg/g. The limit of detection of quercetin was 3.5 μg/g, and the limit of quantification was 12.0 μg/g. Conclusion This method is simple, rapid, and stable, with a simple pre-treatment method, high detection sensitivity, and good stability. It can effectively and accurately detect several phytochemicals, including gallic acid, rutin, ellagic acid, and quercetin, in solid drinks samples simultaneously. It can serve as a reference for quality control of multiple phytochemicals in complex solid drinks.
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