Hilic柱高效液相色谱法检测卵磷脂粉饮品中的磷脂酰胆碱

Determination of phosphatidylcholine in soybean lecithin powder by high performance liquid chromatography with Hilic column

中文关键词:  磷脂酰胆碱  高效液相色谱法  亲水作用Hilic柱

英文关键词:phosphatidylcholine  high performance liquid chromatography  hydrophilic Hilic column

中文摘要:

      目的 建立亲水作用高效液相色谱法用于检测大豆卵磷脂粉饮品中的磷脂酰胆碱。方法 样品中的磷脂酰胆碱用氯仿甲醇溶液(2:1, V:V)提取, 色谱柱为XBridge Hilic BEH (250 mm×4.6 mm, 5 μm), 流动相以乙腈和乙腈水溶液(2:1, V:V)进行梯度洗脱, 检测波长为205 nm, 流量为1.0 mL/min, 以峰面积为指标进行定量分析。结果 大豆磷脂酰胆碱的保留时间为18.04 min, 且在0.009971~0.498600 mg/mL范围内线性关系良好, 相关系数(r2)为0.9994; 方法检出限为7.5 μg/g, 测定结果的相对标准偏差为1.18%, 加标回收率为95.39%~99.52%, 样品溶液24 h内测定结果的相对标准偏差为0.49%。结论 该方法使用亲水作用Hilic柱检测大豆卵磷脂粉饮品中的磷脂酰胆碱, 前处理简单、快速、准确度高、分离度好、检测灵敏, 有效解决高效液相法检测大豆磷脂酰胆碱需要经过氨基固相柱净化处理、分析时间长、操作复杂的问题, 适用于大批量检测大豆卵磷脂粉饮品中的磷脂酰胆碱。

英文摘要:

      Objective To establish a hydrophilic high performance liquid chromatography for detecting phosphatidylcholine in soy lecithin powder beverages. Methods Phosphatidylcholine in the sample was extracted using chloroform methanol solution (2:1, V:V), and the chromatographic column was XBridge Hilic BEH (250 mm×4.6 mm, 5 μm). The mobile phase was gradient eluted with acetonitrile and acetonitrile aqueous solution (2:1, V:V), and the detection wavelength was 205 nm, with a flow rate of 1.0 mL/min. Quantitative analysis was performed using peak area as the indicator. Results The results showed that the retention time of soybean phosphatidylcholine was 18.04 min, and there was a good linear relationship within the range of 0.009971–0.498600 mg/mL, with a correlation coefficient (r2) of 0.9994; the limit of detection of the method was 7.5 μg/g, the relative standard deviation of the determination result was 1.18%, the recovery rate of spiked solution was 95.39%–99.52%, and the relative standard deviation of the determination result within 24 hours of the sample solution was 0.49%. Conclusion This method uses a hydrophilic Hilic column to detect phosphatidylcholine in soy lecithin powder beverages. The pre-treatment is simple, fast, accurate, well separated, and the detection is sensitive. It effectively solves the problems of sample purification, long analysis time, and complex operation required for high performance liquid chromatography detection of soy lecithin choline. It is suitable for large-scale detection of phosphatidylcholine in soy lecithin powder beverages.