胶体金免疫层析法快速检测动物肌肉组织中喹乙醇代谢物

Rapid detection of quinol metabolites in animal muscle tissue by colloidal gold immunochromatography

中文关键词:  喹乙醇  3-甲基喹噁啉-2-羧酸  快速检测  液相色谱-质谱法

英文关键词:quinol  3-methylquinoxaline-2-carboxylic acid  colloidal gold immunochromatography  rapid detection  liquid chromatography-mass spectrometry

中文摘要:

      目的 优化胶体金免疫层析法快速检测动物肌肉组织中喹乙醇代谢物。方法 样品中的喹乙醇残留标志物3-甲基喹噁啉-2-羧酸经盐酸水解、乙腈提取、磷酸缓冲盐溶液复溶, 用于胶体金试纸条的检测。依据GB 31650—2019《食品安全国家标准 食品中兽药最大残留限量》拟定检出限, 筛选符合要求的快速检测产品, 根据《市场监管总局关于规范食品快速检测使用的意见》(国市监食检规〔2023〕1号)对快速检测产品进行评价, 初步确定该方法的性能指标和交叉反应率要求, 并与液相色谱-质谱法进行一致性分析。结果 该方法适用于猪、鸡、鱼和虾肌肉组织的检测, 猪肉的检出限为4 μg/kg, 鸡肉、鱼肉、虾肉的检出限为0.5 μg/kg, 方法灵敏度≥95%、特异性≥95%、假阴性率≤5%、假阳性率≤5%; 卡巴氧代谢物喹噁啉-2-羧酸的交叉反应率≤8.0%, 喹乙醇、卡巴氧、乙酰甲喹、乙酰甲喹代谢物2-(1-羟乙基)-3-甲基喹噁啉-1,4-二氧化物的交叉反应率<0.1%。该方法测定结果与液相色谱-质谱法一致。结论 该方法前处理简单、快速准确, 适用于喹乙醇残留物的快速筛查与确证, 为喹乙醇残留物快速检测的研发与监测提供综合性的技术手段。

英文摘要:

      Objective To optimize a colloidal gold immunochromatographic assay for the rapid detection of quinol metabolites in animal muscle tissues. Methods The quinol residue marker 3-methylquinoxaline-2-carboxylic acid in the sample was hydrolyzed by hydrochloric acid, extracted by acetonitrile, and re-solubilized by phosphate buffer salt solution, and used for the detection of colloidal gold test strips. The limit of detection was formulated based on GB 31650—2019 National standard for food safety-Maximum residue limits of veterinary drugs in food, and the screening of the rapid test products that met the requirements was carried out, and the rapid test products were evaluated according to the Opinions of the general administration of market supervision on the standardization of the use of rapid tests for foods (Guoshiheng Jianshi Food Inspection Regulation 〔2023〕 No.1), which preliminarily determined the performance indexes of the method and the requirement of the cross-reactivity rate, the consistency analysis with liquid chromatography-mass spectrometry was carried out. Results The method was applicable to the detection of pig, chicken, fish and shrimp muscle tissue, the limit of detection of pork was 4 μg/kg, the limit of detection of chicken, fish and shrimp was 0.5 μg/kg, the method sensitivity≥95%, specificity≥95%, false-negative rate≤5%, false-positive rate≤5%; the cross-reactivity rate of the carbachol metabolite quinoxaline-2-carboxylic acid was≤8.0%, the cross-reactivity rate of quinolol, carbacholol and acetylmethoquine, acetylmethoquine metabolite 2-(1-hydroxyethyl)-3-methylquinoxaline-1,4-dioxide was<0.1%. The results of this method were consistent with those of liquid chromatography-mass spectrometry. Conclusion The method is simple, rapid and accurate in pretreatment, suitable for the rapid screening and confirmation of quinol residues, and provides a comprehensive technical means for the research and development and monitoring of quinol residue rapid detection.