长鳍金枪鱼胶原低聚肽抗疲劳作用研究

Study on the anti-fatigue effects of Thunnus alalunga collagen oligopeptides

中文关键词:  长鳍金枪鱼（Thunnus alalunga）  胶原低聚肽  抗疲劳  能量代谢  氧化应激  肌肉保护

英文关键词:Thunnus alalunga  collagen oligopeptides  anti-fatigue  energy metabolism  oxidative stress  muscle protection

中文摘要:

      目的 以长鳍金枪鱼(Thunnus alalunga)鱼皮胶原低聚肽(tuna skin collagen oligopeptides, TSCOP)为原料, 探究TSCOP对小鼠的抗疲劳作用。方法 将TSCOP设置为低[0.2 mg/(g·d)]、中[0.4 mg/(g·d)]、高[0.8 mg/(g·d)] 3个剂量组, 对小鼠连续4周灌胃, 对各组小鼠抗疲劳状态和指标进行评价。结果 TSCOP对小鼠体重、摄食量和机体正常无显著影响, 并能一定程度延长小鼠力竭运动时间。从能量代谢方面看, 高剂量TSCOP可显著提升小鼠体内血糖(P<0.001)和肌糖原水平(P<0.05), 极显著地降低蛋白质代谢产物尿素氮和血氨的积累(P<0.001)。此外, 高剂量组TSCOP可显著降低血清中肌酸激酶和乳酸脱氢酶的含量(P<0.01)。在氧化应激防护方面, 高剂量TSCOP能显著调节机体的氧化/抗氧化系统平衡(P<0.05), 降低丙二醛等脂质过氧化产物的含量。结论 TSCOP不仅能促进骨骼肌原纤维的修复, 还显著提升肌纤维面积、肌质量和肌力量, 全面改善小鼠肌肉的疲劳状态, 显著提升了其运动耐力, 具有显著的抗疲劳功能, 在运动营养、健康保健等领域展现出广泛的应用前景。

英文摘要:

      Objective To investigate the anti-fatigue effects of tuna skin collagen oligopeptides (TSCOP) on mice using TSCOP derived from the skin of Thunnus alalunga. Methods TSCOP was administered at 3 dose levels—low [0.2 mg/(g·d)], medium [0.4 mg/(g·d)], and high [0.8 mg/(g·d)]—via gavage to mice for 4 consecutive weeks. The anti-fatigue status and related indicators of the mice in each group were evaluated. Results TSCOP had no significant impact on the body weight, food intake, or normal physiological functions of the mice, but it did extend the exhaustive exercise time to some extent. From the perspective of energy metabolism, high-dose TSCOP significantly increased blood glucose (P<0.001) and muscle glycogen levels (P<0.05) in the mice, while markedly reducing the accumulation of protein metabolism byproducts such as urea nitrogen and blood ammonia (P<0.001). Additionally, high-dose TSCOP significantly decreased serum levels of creatine kinase and lactate dehydrogenase (P<0.01). In terms of oxidative stress protection, high-dose TSCOP significantly regulated the balance of the oxidative/antioxidant system (P<0.05) and reduced the levels of lipid peroxidation products such as malondialdehyde. Conclusion TSCOP not only promotes the repair of skeletal muscle myofibrils but also significantly increases muscle fiber area, muscle mass, and muscle strength, comprehensively improving the fatigue state of the mice’s muscles and significantly enhancing their exercise endurance. TSCOP exhibits notable anti-fatigue properties and shows broad application potential in fields such as sports nutrition and health care.