周朗君,陈 钦,李秀英.丹磺酰氯柱前衍生-反相高效液相色谱法同时测定水产品中9种生物胺[J].食品安全质量检测学报,2025,16(5):24-31
丹磺酰氯柱前衍生-反相高效液相色谱法同时测定水产品中9种生物胺
Simultaneous determination of 9 kinds of biogenic amines in aquatic products by dansyl chloride precolumn derivatization-reverse phase high performance liquid chromatography
投稿时间:2024-10-18  修订日期:2025-02-14
DOI:
中文关键词:  丹磺酰氯  柱前衍生  反相高效液相色谱法  水产品  生物胺
英文关键词:dansyl chloride  precolumn derivazation  reverse phase high performance liquid chromatography  aquatic product  biogenic amine
基金项目:广州检验检测认证集团有限公司科技项目(2019kj07GZ)
作者单位
周朗君 1. 广州检验检测认证集团有限公司, 2. 国家加工食品质量检验检测中心(广东) 
陈 钦 1. 广州检验检测认证集团有限公司, 2. 国家加工食品质量检验检测中心(广东) 
李秀英 1. 广州检验检测认证集团有限公司, 2. 国家加工食品质量检验检测中心(广东) 
AuthorInstitution
ZHOU Lang-Jun 1. Guangzhou Inspection Testing and Certification Group Co., Ltd., 2. National Quality Testing Center for Processed Food (Guangdong) 
CHEN Qin 1. Guangzhou Inspection Testing and Certification Group Co., Ltd., 2. National Quality Testing Center for Processed Food (Guangdong) 
LI Xiu-Ying 1. Guangzhou Inspection Testing and Certification Group Co., Ltd., 2. National Quality Testing Center for Processed Food (Guangdong) 
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中文摘要:
      目的 建立丹磺酰氯柱前衍生-反相高效液相色谱法同时检测水产品中9种生物胺的检测方法。方法 采用5%三氯乙酸水溶液提取, 10 mg/mL丹磺酰氯丙酮溶液衍生, 氨水终止衍生反应, 乙腈定容获得9种生物胺的丹磺酰氯衍生物待测溶液。试样溶液通过反相液相色谱检测, 以乙腈和水为流动相梯度洗脱, 采用C18色谱柱分离, 紫外检测器在254 nm下检测。结果 水产品中9种生物胺的定量限在1.08~7.59 mg/kg, 加标回收率在85.2%~106.7%, 精密度在1.5%~4.9%。采用所建立的方法测定广州市售的50批次高组胺鱼类, 结果发现, 广州市售的高组胺鱼类质量整体较好, β-苯乙胺和尸胺相对其他生物胺有较高检出率, 但含量均低于35 mg/kg, 色胺、组胺、章鱼胺、亚精胺、精氨均为未检出。结论 该方法快速、简便、灵敏度高, 适用于水产品中9种生物胺的批量样品测定。
英文摘要:
      Objective To establish a detection method for simultaneously detecting 9 kinds of biogenic amines in aquatic products using the dansyl chloride precolumn derivazation-reverse phase high performance liquid chromatography. Methods The samples were extracted with a 5% trichloroacetic acid solution and derivatized with a 10 mg/mL dansyl chloride acetone solution. The derivatization reaction was terminated with ammonia, and the resulting solution containing the dansyl chloride derivatives of the 9 kinds of biogenic amines was made up to volume with acetonitrile. The sample solution was analyzed by reverse phase liquid chromatography using a C18 column, with a mobile phase gradient of acetonitrile and water, and detected at 254 nm using a ultraviolet detector. Results The limits of quantitation of the 9 kinds of biogenic amines in aquatic products ranged from 1.08 to 7.59 mg/kg, with recovery rates ranging from 85.2% to 106.7% and precision ranging from 1.5% to 4.9%. The established method was used to analyze 50 batches of high-histamine fish sold in Guangzhou. The results revealed that the overall quality of the high-histamine fish in Guangzhou was good. β-phenylethylamine and cadaverine had relatively higher detection rates compared to other biogenic amines, but their content was all below 35 mg/kg. Histamine, putrescine, spermidine, agmatine, and arginine were not detected. Conclusion The method is fast, simple, and highly sensitive, making it suitable for batch sample determination of the 9 kinds of biogenic amines in aquatic products.
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