合相色谱法定量检测蔬菜中的叶绿素含量

Quantitative determination of chlorophyll content in vegetables by convergence chromatography

中文关键词:  合相色谱  叶绿素  分析方法开发  羽衣甘蓝  芥菜  雪菜  梅干菜  

英文关键词:convergence chromatography  chlorophyll  kale  fresh mustard greens  pickled mustard  preserved pickled mustard

中文摘要:

      目的 建立合相色谱法(convergence chromatography, CC)测定蔬菜中叶绿素a和叶绿素b的含量。方法 采用索氏抽提法对蔬菜及其制品中的叶绿素进行高效提取, 并使用超高效合相色谱仪结合紫外-可见检测器进行分离与检测。结果 通过对不同溶剂稳定性的对比, 选择了石油醚作为提取溶剂, 并优化了提取条件。经验证, 检出限为0.02 mg/kg, 定量限为0.06 mg/kg, 线性范围为0.06~800.00 mg/kg, 加标回收率为99%~102%, 真实样品相对标准偏差2.2%~14.0%。通过对真实样品, 包括羽衣甘蓝、鲜芥菜、雪菜、梅干菜、蒸煮梅干菜、水煮梅干菜的检测精密度考察并与现行标准方法的交叉验证, 证明了该方法的可靠性和准确性。结论 本研究建立了一种基于CC的检测蔬菜中叶绿素a和叶绿素b的方法, 具有较高的灵敏度、较宽的线性范围和良好的特异性和精密度。该方法不仅适用于实验室研究, 也具备实际应用潜力。

英文摘要:

      Objective To establish a method for the determination of chlorophyll a and chlorophyll b content in vegetables by convergence chromatography (CC). Methods The Soxhlet extraction method was used for efficient extraction of chlorophyll from vegetables and their products, followed by separation and detection using an ultra-performance convergence chromatography system coupled with a ultraviolet-visible detector. Results Through comparison of the stability of different solvents, petroleum ether was selected as the extraction solvent, and the extraction conditions were optimized. The method was validated with a limit of detection of 0.02 mg/kg, a limit of quantification of 0.06 mg/kg, a linear range of 0.06–800.00 mg/kg, a spike recovery rate of 99%–102%, and a relative standard deviation of 2.2%–14.0% for real samples. The reliability and accuracy of the method were confirmed through precision testing of real samples, including kale, fresh mustard greens, pickled mustard greens, dried pickled mustard greens, steamed dried pickled mustard greens, and boiled dried pickled mustard greens, as well as cross-validation with existing standard methods. Conclusion This study establishes a CC-based method for detecting chlorophyll a and chlorophyll b in vegetables, demonstrating high sensitivity, a wide linear range, good specificity, and precision. The method is not only suitable for laboratory research but also has practical application potential.