液相色谱-串联质谱法测定酒类饮品中爱德万甜含量

Determination of advantame in alcoholic beverages by liquid chromatography-tandem mass spectrometry

中文关键词:  爱德万甜  酒类饮品  高灵敏度  液相色谱-串联质谱法

英文关键词:advantame  alcoholic beverages  high sensitivity  liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立一种液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)检测酒类饮品中爱德万甜的高灵敏度分析方法。方法 酒样通过超纯水进行10倍稀释后过膜进样, 使用Kinetex? Biphenyl 100 ?色谱柱(100 mm×3.0 mm, 2.6 μm), 以水和甲醇为流动相梯度洗脱分离, 采用电喷雾离子源(electrospray ionization, ESI), 在正离子扫描多反应监测(multiple reaction monitoring, +MRM)模式下检测, 外标法定量。结果 爱德万甜在0.03~40.00 μg/L质量浓度范围内呈现出优良的线性关系, 相关系数达0.9999, 检出限为0.10 μg/kg, 定量限为0.30 μg/kg, 在低、中低、中高、高4个不同浓度加标水平下的平均回收率为80.8%~109.8%, 相对标准偏差为2.7%~12.5% (n=6)。结论 该方法检测灵敏度高, 操作简单, 准确可靠, 可用于酒类饮品中爱德万甜的痕量分析及风险评估, 为科学监管酒类市场提供有力的技术支撑。

英文摘要:

      Objective To establish a highly sensitive analytical method for the determination of advantame in alcoholic beverages by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods The wine samples were diluted 10 times with ultrapure water, filtered through a membrane, and then injected. A Kinetex? Biphenyl 100 ? chromatographic column (100 mm×3.0 mm, 2.6 μm) was used, with water and methanol as the mobile phase for gradient elution and separation. An electrospray ionization source (ESI) was adopted for detection in the positive ion scan multiple reaction monitoring (+MRM) mode, and external standard method was used for quantification. Results Advantame exhibited an excellent linear relationship within the mass concentration range of 0.03 to 40.00 μg/L, with a correlation coefficient of 0.9999. The limit of detection was 0.10 μg/kg, the limit of quantification was 0.30 μg/kg. The average recoveries at the 4 different spiked levels of low, medium-low, medium-high and high were 80.8% to 109.8%, and the relative standard deviation was 2.7% to 12.5% (n=6). Conclusion This method has high detection sensitivity, is simple to operate, accurate and reliable, and can be used for the trace analysis and risk assessment of advantame in alcoholic foods, providing strong technical support for scientific supervision of the alcoholic beverage market.