高效液相色谱法同时测定7种药食同源物质功能成分

Simultaneous determination of functional components of 7 kinds of medicinal and food homologous substances by high performance liquid chromatography

中文关键词:  高效液相色谱法  同时测定  药食同源  功能成分  检测

英文关键词:high performance liquid chromatography  simultaneous determination  medicinal and food homologous  functional components

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时测定药食同源性物质葛根、槐花、金银花、肉苁蓉、罗汉果、人参、枳椇子提取物中功能成分的检测方法。方法 采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm, 5 μm), 以乙腈-0.1%磷酸为流动相梯度洗脱, 流速0.6 mL/min, 柱温30 ℃, 检测波长210 nm, 进样量5 μL, 测定葛根素、芦丁、绿原酸、松果菊苷、毛蕊花糖苷、罗汉果甜苷V、人参皂苷Re及二氢杨梅素的含量。结果 8种功能成分在各自浓度范围内线性关系良好, 相关系数(r2)>0.999, 检出限为0.02~1.88 mg/L, 定量限为0.08~3.62 mg/L, 精密度实验结果为相对标准偏差(relative standard deviation, RSD)<3%, 平均加标回收率为95.49%~109.87%。结论 该方法简便快速, 准确度高, 可作为上述7种药食同源性物质功能成分的定性定量分析方法。

英文摘要:

      Objective To establish a method for the simultaneous determination of functional components in extracts from 7 kinds of medicinal and food homologous substances: Pueraria lobata, Sophora japonica, Lonicera japonica, Cistanche deserticola, Siraitia grosvenorii, Panax ginseng and Hovenia dulcis by high performance liquid chromatography (HPLC). Methods The analysis was conducted using an Agilent Eclipse XDB-C18 column (4.6 mm× 250 mm, 5 μm) with a mobile phase gradient elution of acetonitrile-0.1% phosphoric acid at a flow rate of 0.6 mL/min, a column temperature of 30 ℃, a detection wavelength of 210 nm, and an injection volume of 5 μL. This method was employed to quantify the content of puerarin, rutin, chlorogenic acid, echinacoside, verbascoside, mogroside V, ginsenoside Re and dihydromyricetin. Results The results demonstrated that 8 kinds of functional components exhibited excellent linear relationships within their respective concentration ranges, with correlation coefficients (r2) exceeding 0.999. The limits of detection ranged from 0.02 to 1.88 mg/L, and the limits of quantification ranged from 0.08 to 3.62 mg/L. The precision experiment results showed that the relative standard deviation (RSD) was less than 3%, the average recoveries of spiked samples ranged from 95.49% to 109.87%. Conclusion This method is simple, rapid and highly accurate, making it suitable for the qualitative and quantitative analysis of the functional components in the aforementioned 7 kinds of medicinal and food homologous substances.