硇洲岛海岸栽培礁膜重金属与毒性检测及其食用安全性研究

Research on heavy metal and toxicity testing and edible safety of cultivated Monostromanitdum on the coast of Naozhou Island

中文关键词:  硇洲岛  礁膜  重金属  急性经口毒性实验  食用安全性

英文关键词:Naozhou Island  Monostromanitdum  heavy metals  acute oral toxicity test  edible safety

中文摘要:

      目的 评价硇洲岛海岸海水养殖礁膜的食用安全性。方法 以4种采集时间不同的礁膜为研究对象, 采用微波消解法、电感耦合等离子体质谱和原子荧光光谱法对礁膜样品中的Cr、As、Pb、Hg、Cd 5种重金属含量进行分析, 并进行微生物指标测定, 采用小鼠急性经口毒性实验, 通过分析小鼠的饮水、饮食、体重、脏器系数、死亡率及外观变化等指标来评价礁膜的食用安全性。结果 样品中Cr、As、Pb、Hg含量均远超过国家标准规定食用性安全含量, 分别为限定值的29.20、12.12、3.59、3.70倍。样品菌落总数均超过50 CFU/g。灌胃礁膜组小鼠出现死亡现象, 脏器系数除肝脏外其他脏器均出现升高, 且外观变化符合重金属中毒表现。结论 食用未经加工处理的礁膜存在安全风险, 直接食用安全性较低。

英文摘要:

      Objective To evaluate the edible safety of marine aquaculture Monostromanitdum along the coast of Naozhou Island. Methods Using 4 kinds of different collection times of Monostromanitdum as research objects, microwave digestion, inductively coupled plasma mass spectrometry, and atomic fluorescence spectroscopy were used to analyze the content of 5 kinds of heavy metals, Cr, As, Pb, Hg, and Cd, in Monostromanitdum samples, and microbial indicators were determined. Using acute oral toxicity experiments on mice, the food safety of Monostromanitdum was evaluated by analyzing indicators such as drinking water, diet, body weight, organ coefficient, mortality rate, and appearance of mice. Results The content of Cr, As, Pb, and Hg in the sample far exceeded the food safety content specified by the national standard, with the maximum being 29.20, 12.12, 3.59 and 3.70 times the limit values, respectively. The total number of bacterial colonies in the samples exceeded 50 CFU/g. The mice in the group administered with Monostromanitdum by gavage showed signs of death, with organ coefficients increasing in all organs except for the liver, and the appearance changes consistent with heavy metal poisoning. Conclusion There are safety risks associated with consuming unprocessed Monostromanitdum, and the safety of direct consumption is relatively low.