功效型米粉对高脂诱导高血糖小鼠的降血糖作用研究

Study on the hypoglycemic effect of functional rice flour on hyperglycemia induced by high fat in mice

中文关键词:  药食同源  功效性米粉  高血糖模型  肠道菌群  降血糖

英文关键词:homology between medicine and food  functional rice flour  hyperglycemia model  intestinal microbiota  hypoglycemic

中文摘要:

      目的 探索功效型米粉对高脂诱导高血糖小鼠的降血糖作用。方法 通过使用高脂饲料联合多次腹腔注射小剂量链脲佐菌素的方法,建立高血糖小鼠模型。用黄芪粉、山药粉、葛根粉、薏苡仁粉、沙棘粉和荞麦粉制备功效型米粉,然后对小鼠进行米粉干预作用试验。测定干预作用完成时各组小鼠空腹血糖、胰岛素水平、葡萄糖耐量、血清中的血脂四项指标及总胆汁酸的含量,检测米粉干预下高血糖小鼠肠道菌群的变化。结果 与模型组相比,米粉组小鼠体重、肝重及肝体比均显著降低(P<0.01)；甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平显著降低(P<0.01)；空腹血糖值、空腹胰岛素水平、胰岛素抵抗均显著降低(P<0.01)；葡萄糖耐量水平显著增强(P<0.01)；可明显回调血清和粪便总胆汁酸含量,提升肝脏总胆汁酸含量；小鼠肠道的厚壁菌门(Firmicutes)丰度有所减少,拟杆菌门(Bactercidetes)的丰度增加,改善失调的肠道菌群。结论 功效型米粉能通过改善体内脂代谢紊乱、增强胆汁酸代谢通路、调节肠道菌群来降低高脂诱导的高血糖小鼠的血糖。

英文摘要:

      ABSTRACT: Objective To explore the hypoglycemic effect of functional rice flour on hyperlipidemia-induced hyperglycemic mice. Methods By using high-fat diet combined with multiple intraperitoneal injections of small doses of streptozotocin, a hypoglycemic mouse model was established. The functional rice flour was prepared with astragalus root powder, yam powder, kudzu root powder, coix seed powder, seabuckthorn powder and buckwheat powder, and then the intervention effect of rice flour was tested in mice. Fasting blood glucose, insulin level, glucose tolerance, the indexes of triglyceride, 4 items of blood lipids and the content of total bile acids of mice in each group were measured when the intervention was completed, the changes of intestinal flora in hyperglycemic mice under the intervention of rice flour were detected. Results Compared with the model group, the body weight in the rice flour group was significantly reduced (P<0.01).The levels of total cholesterol, total triglyceride and low-density lipoprotein were significantly reduced (P<0.01). Fasting blood glucose levels, fasting insulin levels, and insulin resistance were significantly reduced (P<0.01). The glucose tolerance level was significantly enhanced (P<0.01). The total bile acid content in serum and feces were significantly reduced, and the total bile acid content in the liver was significantly increased. The abundance of Firmicutes in the mouse gut was decreased, while the abundance of Bacteroidetes was increased, the dysregulated gut microbiota was improved. Conclusion Functional rice flour can reduce the blood glucose of hyperlipidemia-induced hyperglycemic mice by improving the disorder of lipid metabolism, enhancing bile acid metabolic pathway and regulating intestinal flora.