宁夏地区部分食源性耐甲氧西林金黄色葡萄球菌分子分型、耐药特征及毒力基因分析

Molecular typing, antibiotic resistance characteristics, and virulence gene analysis of some food-borne methicillin-resistant Staphylococcus aureus in Ningxia

中文关键词:  MRSA  分型特征  耐药特征  毒力基因

英文关键词:methicillin-resistant Staphylococcus aureus  typing characteristic characteristics  antimicrobial resistance characteristics  virulence genes

中文摘要:

      目的 了解宁夏地区部分食源性耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureu, MRSA)的分型特征和耐药特征以及与毒力基因携带情况。方法 收集宁夏地区部分食品风险监测分离的MRSA, 分别进行药物敏感试验、脉冲场凝胶电泳(pulsed-fiedld gel electrophoresis, PFGE)以及全基因组测序分析。结果 9株来自食源性的MRSA菌株PFGE分型分为3簇8种型别其源性都在85%以上, 全基因组测序结果ST分型分为4种ST型, 6株为ST59, 其余3株分别为ST3355、ST7、ST965。MRSA均为多重耐药, 携带耐药基因以及毒力基因也各不相同, 导致的耐药表型也存在差异。结论 食源性分离MRSA基因组中存在大量与耐药和致病相关基因, 这些基因给人类健康带来巨大威胁, 因此持续监测并采取有效措施降低食品中MRSA的污染水平, 以确保食品安全。

英文摘要:

      Objective To investigate the type and drug resistance characteristics and virulence gene carrying of some food-borne methicillin-resistant Staphylococcus aureu (MRSA) in Ningxia. Methods MRSA isolates from some food risk monitoring in Ningxia were collected and subjected to drug susceptibility testing, pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing analysis respectively. Results PFGE typing of 9 food-borne MRSA strains was divided into 3 clusters and 8 kinds of types, all of which were more than 85% provenance. ST typing of the whole genome sequencing results was divided into 4 kinds of ST types, 6 strains were ST59, and the other 3 strains were ST3355, ST7 and ST965, respectively. MRSA were all multi-drug resistant, which carrying different resistance genes and virulence genes, resulting in different drug resistance phenotypes. Conclusion Foodborne MRSA isolates have a large number of genes related to antibiotic resistance and pathogenicity, which pose a significant threat to human health. Therefore, it is of great importance to continuously monitor and take effective measures to reduce the contamination level of MRSA in food to ensure food safety.