胶体金检测卡测定小麦和玉米中伏马毒素和T-2/HT-2毒素的方法学联合验证

Interlaboratory validation of colloidal gold detection card for detecting fumonisin and T-2/HT-2 toxin in Triticum aestivum L. and Zea mays

中文关键词:  胶体金  方法联合验证  小麦  玉米

英文关键词:colloidal gold  method joint verification  Triticum aestivum L.  Zea mays

中文摘要:

      目的 建立胶体金检测卡法测定小麦和玉米中伏马毒素和T-2/HT-2毒素的分析方法。方法 在6家实验室间开展联合验证, 评价了方法的检出限、定量限、精密度以及准确性。结果 伏马毒素和T-2/HT-2毒素方法的检出限分别为150 μg/kg和5 μg/kg, 定量限分别为450 μg/kg和11 μg/kg。当小麦、玉米中伏马毒素含量在426.39~3897.22 μg/kg时, 得出方法重复性标准差Sr和再现性标准差SR与伏马毒素含量m的关系式, 即重复性标准差Sr=1.478m0.600, 再现性标准差SR=4.269m0.591; 当小麦、玉米中T-2/HT-2毒素含量在426.39~3897.22 μg/kg时, 重复性标准差Sr和再现性标准差SR与T-2/HT-2毒素含量m的关系式分别为: 重复性标准差Sr=3.1901m0.218, 再现性标准差SR=0.2507m0.492(小麦基质); 重复性标准差Sr=0.8357m0.333, 再现性标准差SR=1.1231m0.585(玉米基质)。在3个浓度水平下进行加标回收实验, 伏马毒素的回收率在95.42%~101.90%之间, T-2/HT-2毒素的回收率在83.30%~118.20%之间。与标准方法进行配对t检验, 结果无显著性差异。结论 本方法准确性好, 可以广泛推广用于小麦、玉米中伏马毒素、T-2/HT-2毒素含量的快速定量检测。

英文摘要:

      Objective To establish a rapid colloidal gold card method for the determination of fumonisin and T-2/HT-2 toxin in Triticum aestivum L. and Zea mays. Methods The limit of detection, limit of quantitative, precision and accuracy of the method were evaluated in 6 laboratories. Results The limits of detection of fumonisin and T-2/HT-2 toxin methods were 150 μg/kg and 5 μg/kg, respectively. The limits of quantitation were 450 μg/kg and 11 μg/kg, respectively. When the fumonin content in Triticum aestivum L. and corn ranged from 426.39 to 3897.22 μg/kg, the relationship between the method repeatability standard deviation Sr and reproducibility standard deviation SR and fumonin content was obtained, namely, repeatability standard deviation Sr=1.478m0.600 and reproducibility standard deviation SR=4.269m0.591. When the T-2/HT-2 toxin content in Triticum aestivum L. and Zea mays ranged from 426.39 to 3897.22 μg/kg, the relation between repeatability standard deviation Sr and reproducibility standard deviation SR and T-2/HT-2 toxin content m was as follows: Repeatability standard deviation Sr=3.1901m0.218, reproducibility standard deviation SR=0.2507m0.492 (Triticum aestivum L. matrix). Repeatability standard deviation Sr=0.8357m0.333, reproducibility standard deviation SR=1.1231m0.585 (Zea mays matrix). The recoveries of fumonisin and T-2/HT-2 toxin ranged from 95.42% to 101.90% and 83.30% to 118.20% respectively. There was no significant difference between the paired t test and the standard method. Conclusion The method has good accuracy and can be widely used for rapid and quantitative determination of fumonisin and T-2/HT-2 toxin in Triticum aestivum L. and Zea mays.