表面增强拉曼光谱法快速检测食源性致病菌研究进展

Research progress on rapid analysis of foodborne pathogen bacteria in food by surface-enhanced Raman spectroscopy

中文关键词:  表面增强拉曼光谱  样品制备  快速检测  食源性致病菌  研究进展

英文关键词:surface-enhanced Raman spectroscopy  sample preparation  rapid detection  foodborne pathogen bacteria

中文摘要:

      食源性致病菌作为食品和水源直接或间接污染物, 已成为全球范围内严峻的食品安全问题之一。因此, 研究食源性致病菌快速、准确、可靠检测方法对食品安全监管具有重要意义。表面增强拉曼光谱(surface-enhanced Raman spectroscopy, SERS)技术具有灵敏、快速、无损等优点, 有望应用于食源性致病菌的快速检测, 但为消除复杂食品基质对SERS检测的干扰, 需结合有效的样品前处理技术以提高SERS检测的准确度。同时针对菌体SERS信号的相似性和拉曼光谱数据处理的复杂性, 需引入化学计量学辅助方法, 以有效提高数据处理的效率和准确性。本文综述了SERS在食源性致病菌快速检测中的研究进展, 包括食品中致病菌检测样品制备方法、SERS快速定性定量方法研究进展, 总结了用于食源性致病菌快速检测的无标签表面增强拉曼和有标签表面增强拉曼, 展望了表面增强拉曼光谱快速检测技术研究的发展方向。

英文摘要:

      Foodborne pathogen bacteria, which are direct or indirect contaminants of food and water and easily cause diseases, have emerged as one of the most critical food safety issues worldwide. Therefore, the development of rapid, accurate, and reliable analytical detection techniques for foodborne pathogen bacteria is of paramount importance for food safety regulation. Surface-enhanced Raman spectroscopy (SERS) offers several advantages, including high sensitivity, rapid detection, and non-destructive analysis, making it suitable for the development of integrated methods and portable devices. SERS has been applied to the rapid detection of various samples and scenarios. However, interference caused by complex food matrices remains a significant challenge, necessitating the combination of SERS with sample pretreatment techniques to enhance detection accuracy. Additionally, the similarity of bacterial signals and the complexity of spectral data require advanced chemometric processing and the use of labeled probes. This review, reviewed the research progress of surface-enhanced Raman spectroscopy in the rapid analysis of foodborne pathogen bacteria, included the sample preparation methods, the rapid qualitative and quantitative SERS methods of foodborne pathogen bacteria in food. Introduced the label-free and labeled Raman spectroscopy for rapid analysis of foodborne pathogen bacteria. The development direction of Raman spectroscopy rapid detection technique was prospected.