垂钓饵料中地西泮在鲫鱼体内代谢及组织分布研究

Study on the metabolism and tissue distribution of diazepam in Carassius auratus from angling bait

中文关键词:  垂钓渔业  地西泮  鲫鱼  饵料  代谢

英文关键词:diazepam  Carassius auratus  bait  metabolism

中文摘要:

      目的 研究鲫鱼摄食含地西泮(diazepam, DZP)的阳性饵料后, DZP在其体内的组织分布及代谢规律。方法 选择鲫鱼作为研究对象, 使用含DZP的阳性饵料进行灌胃给药, 并在给药后1 h至456 h内, 定时对鱼鳞、鱼皮、肌肉、血浆、鳃、肠、肝脏、胆、性腺及脑等组织进行取样。采用高效液相色谱-串联高分辨质谱技术, 检测各组织中DZP及其代谢物去甲地西泮(nordazepam, NZP)、替马西泮(temazepam, TZP)、奥沙西泮(oxazepam, OZP)的残留量, 并分析其在组织中的分布特征和代谢规律。结果 摄食含DZP的阳性饵料后, DZP在鲫鱼体内迅速达到较高的残留量, 并在456 h后仍维持在高水平, 主要集中在性腺、肝脏和胆中, 尤其在性腺中残留量显著, 其主要代谢物为NZP和TZP。结论 DZP在鲫鱼体内主要以原型物形态存在, 并且容易在性腺中富集。DZP及其主要代谢物在鲫鱼体内的代谢周期较长, 消费者食用使用含DZP饵料捕获的水产品可能存在食品安全问题和健康风险。

英文摘要:

      Objective To study the tissue distribution and metabolic regulation of diazepam (DZP) in Carassius auratus after feeding with positive bait containing DZP. Methods Carassius auratus was selected as the study object and exposed to DZP-enriched bait through gavage, and tissue samples including scales, skin, muscle, plasma, gills, intestines, liver, gallbladder, gonads, and brain were collected at specific intervals ranging from 1 h to 456 h post-exposure. High performance liquid chromatography coupled with tandem high-resolution mass spectrometry was employed to detect DZP and its metabolites—nordazepam (NZP), temazepam (TZP), and oxazepam (OZP)—in these tissues, analyze their distribution and metabolic patterns. Results Following exposure to DZP-enriched bait, DZP rapidly accumulated in Carassius auratus tissues, with substantial residues persisting for up to 456 h. The highest concentrations were primarily found in the gonads, liver and gallbladder, with the gonads consistently showing elevated levels. NZP and TZP emerged as the primary metabolites. Conclusion DZP predominantly exists in its prototype form in Carassius auratus and tends to accumulate in the gonads. The prolonged metabolic cycle of DZP and its metabolites in Carassius auratus indicates potential food safety and health risks for consumers of aquatic products caught with DZP-containing bait.