β-烟酰胺单核苷酸对高脂血症大鼠肝脏的保护作用研究

Protective effect of β-nicotinamide mononucleotide on the liver of hyperlipidemic rats

中文关键词:  β-烟酰胺单核苷酸  高脂血症模型  降血脂  护肝

英文关键词:β-nicotinamide mononucleotide  hyperlipidemia model  lipid-lowering  liver protection

中文摘要:

      目的 探究β-烟酰胺单核苷酸(nicotinamide mononucleotide, NMN)对高脂血症模型大鼠的降脂和对肝脏的保护作用。方法 50只雄性SD大鼠, 随机分为正常组、模型组和NMN低、中、高剂量组(10、50、250 mg/kg), 每组10只, 正常组给予维持饲料, 其余各组给予高脂饲料建立高脂模型, 造模成功后分别进行灌胃干预。干预5周后, 检测血清中总胆固醇(total cholesterol, TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白(low density lipoprotein cholesterol, LDL-c)及高密度脂蛋白(high densitylipoprotein cholesterol, HDL-c)、血糖(glucose, GLU)、碱性磷酸酶(alkaline phosphatase, ALP)、丙氨酸氨基转移酶(alanine aminotransferase, ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase, AST)、肌酐(creatinine, CREA)水平, 并结合病理观察肝脏组织学变化和进行非酒精性脂肪肝活动度评分(nonalcoholic fattyliver disease activity score, NAS)评分。结果 与正常组相比, 模型组大鼠体重增加明显, 其血清中的TG、TC、LDL-c和ALT含量极显著上升(P<0.01), AST和ALP含量显著上升(P<0.05), HDL-c含量显著降低(P<0.05), GLU和CREA无显著变化。与模型组相比, 低中剂量组TG、TC、ALT显著降低(P<0.05), 中剂量组LDL-c极显著降低(P<0.01); 高剂量组TG、AST和ALT含量显著降低(P<0.05)而TC和LDL-c极显著降低(P<0.01); HDL-c、ALP、GLU和CREA无显著差异。与模型组相比, 低中高剂量组对大鼠的肝细胞脂肪变性, 炎症浸润均有改善, 中高剂量组的NAS总分降低且有统计学意义。结论 NMN能降低高脂血症SD大鼠的血脂水平, 并对肝脏有一定的保护作用。

英文摘要:

      Objective To investigate the lipid-lowering and liver protective effects of β-nicotinamide mononucleotide (NMN) on hyperlipidemic model rats. Methods The 50 male SD rats were randomly divided into a normal group, a model group and low, medium and high dose groups of NMN (10, 50, 250 mg/kg), with 10 rats in each group. The normal group was given maintenance feed, while the other groups were given high-fat feed to establish a high-fat model. After successful modeling, gastric lavage intervention was performed in each group. After 5 weeks of intervention, serum biochemical indicators and blood lipids were measured, including total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c), high densitylipoprotein cholesterol (HDL-c) levels, glucose (GLU), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA) levels, combined with pathological observation of liver histological changes and nonalcoholic fattyliver disease activity score (NAS) of liver tissues was evaluated. Results Compared with the normal group, the body weight of the model group rats increased significantly, and the levels of TG, TC, LDL-c and ALT (P<0.01) in their serum increased significantly, AST and ALP levels significantly increased (P<0.05) The level of HDL-c (P<0.05) decreased significantly, while GLU and CREA showed no significant changes. Compared with the model group, the TG, TC and ALT levels in the low and medium dose groups were significantly reduced (P<0.05), the LDL-c in the medium dose group was significantly reduced (P<0.01). The TG, AST and ALT content in the high-dose group was significantly reduced (P<0.05). However, TC and LDL-c were significantly reduced (P<0.01); there was no significant difference in HDL-c, ALP, GLU and CREA. Compared with the model group, the low, medium, and high dose groups improved hepatic steatosis and inflammatory infiltration in rats, and the total score of NAS in the medium and high dose groups was reduced and showed statistical significance. Conclusion NMN can reduce the blood lipid levels in hyperlipidemic SD rats and has a certain protective effect on the liver.