超高效液相色谱-串联质谱法评价果蔬中77种农药的基质效应

Evaluation of matrix effects of 77 kinds of pesticides in fruits and vegetables by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  超高效液相色谱-串联质谱法  农药残留  校正曲线  基质效应

英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  pesticide residue  calibration curve  matrix effect

中文摘要:

      目的 采用QuEChERS-超高效液相色谱-串联质谱法评价苦瓜、瓠瓜、小南瓜、茄子、菜心、蕹菜、豇豆、小白菜、芥菜、马齿苋和番木瓜等11种果蔬中77种农药残留的基质效应。方法 采用乙腈稀释混合标准使用液测得结果绘制工作曲线, 果蔬样品经乙腈提取后得到的空白基质液稀释混合标准使用液测得结果绘制工作曲线; 通过两个不同基质的曲线斜率比值法来评价基质效应。结果 不同的农药在不同的基质中产生不同的基质效应, 77种农药中有50%以上的农药在瓠瓜、苦瓜、菜心、豇豆、茄子、番木瓜、小南瓜、蕹菜基质中表现为基质增强效应; 氧乐果在番木瓜中无基质效应影响; 77种农药中有80%以上的农药在瓠瓜、苦瓜、豇豆、茄子、番木瓜、马齿苋、小南瓜、蕹菜基质中表现为弱基质效应。苦瓜、芥菜、菜心、小白菜、马齿苋、蕹菜基质中均有农药表现强基质效应。结论 对于检测在苦瓜、瓠瓜、小南瓜、茄子、菜心、蕹菜、豇豆、小白菜、芥菜、马齿苋和番木瓜中均表现为弱基质效应的农药, 可采用乙腈溶剂或其中某一种基质提取液为溶剂匹配校正曲线进行定量, 而表现为中等基质效应和强基质效应的农药则采用本体基质或有相同基质效应的基质进行替代匹配校正曲线。通过该研究应用于果蔬检测中能快速高效的选择合适的空白基质处理校准曲线, 提高检测数据的准确性。

英文摘要:

      Objective To evaluate the matrix effects of 77 kinds of pesticides in 11 kinds of fruits and vegetables such as balm gourd, gourd gourd, small pumpkin, eggplant, cabbage, water spinach, cowpea, Chinese cabbage, mustard, purslane and papaya by QuEChERs-ultra performance liquid chromatography-series mass spectrometry. Methods The working curve was drawn with the results obtained by the acetonitrile dilution mixing standard, and the working curve was drawn with the results obtained by the liquid dilution mixing standard after the extraction of fruit and vegetable samples by the acetonitrile. The matrix effect was evaluated by the curve slope ratio method of two different matrices. Results Different pesticides produced different matrix effects in different matrices. More than 50% of 77 kinds of pesticides showed matrix enhancement effects in baby marrow, bitter gourd, Chinese cabbage, cowpea, eggplant, papaw, tiny pumpkin, water spinach matrices. Omethoate in papaw matrix showed no matrix effect. More than 80% of 77 kinds of pesticides showed weak matrix effects in calabash gourd, bitter gourd, long beans, papaya, marigold, small pumpkin, and water spinach matrices. Strong matrix effects were observed for pesticides in bitter gourd, mustard greens, bok choy, Chinese cabbage, purslane, and water spinach substrates. Conclusion For the pesticides that shows weak matrix effect in bitter gourd, gourd gourd, small pumpkin, eggplant, cabbage heart, water spinach, cowpea, Chinese cabbage, mustard vegetable, purslane and papaya, acetonitrile solvent or one of the matrix extract is used as the solvent matching correction curve for quantitative determination. For the pesticides with medium and strong matrix effects, the main matrix or matrix with the same matrix effects are used to substitute the calibration curves. The application of this research in fruit and vegetable detection can quickly and efficiently select the appropriate blank matrix to process the calibration curve and improve the accuracy of the detection data.