松花粉发酵产物的安全性评价

Safety evaluation of pine pollen fermentation products

中文关键词:  急性毒性  遗传毒性  28 d经口毒性  精母细胞染色体畸变

英文关键词:acute toxicity  genotoxicity  28-day oral toxicity  spermatocyte chromosomal aberration

中文摘要:

      目的 研究松花粉发酵产物安全性, 对其进行毒理学评价。方法 通过小鼠急性经口毒性试验、细菌回复突变试验、小鼠红细胞微核试验、小鼠精母细胞染色体畸变试验、大鼠28 d经口毒性试验系统地研究松花粉发酵产物的安全性。结果 松花粉发酵产物急性经口毒性试验雌雄小鼠最大耐受剂量大于15 g/(kg·BW), 属于实际无毒级别; 细菌回复突变试验、哺乳动物红细胞微核试验及小鼠精母细胞染色体畸变试验等3项遗传毒性检测结果均为阴性; 28 d经口毒性试验中, 0.625、1.250、2.500 g/(kg·BW) 3个剂量组大鼠的体质量、进食量、血液学、血生化等指标, 无异常变化。结论 在本研究条件和剂量下, 松花粉发酵产物无急性毒性、遗传毒性, 其雌、雄大鼠28 d经口毒性无可见有害作用剂量值至少为2.5 g/(kg·BW)。因此, 松花粉发酵产物具有食用安全性。

英文摘要:

      Objective To study the safety of pine pollen fermentation products and evaluate their toxicology. Methods The safety of pine pollen fermentation products was systematically studied through acute oral toxicity test in mice, bacterial reverse mutation test, red blood cell micronucleus test in mice, spermatocyte chromosomal aberration test in mice, and 28-day oral toxicity test in rats. Results The acute oral toxicity test of pine pollen fermentation products in female and male mice showed that the maximum tolerated dose was greater than 15 g/(kg·BW), which was classified as practically non-toxic; the results of 3 genotoxicity tests, including bacterial reverse mutation test, mammalian red blood cell micronucleus test, and mouse spermatocyte chromosomal aberration test, were all negative; in the 28-day oral toxicity test, no abnormal changes were observed in the body weight, food intake, hematology, and blood biochemistry indicators of rats in the 3 dose groups of 0.625, 1.250, and 2.500 g/(kg·BW). Conclusion Under the experimental conditions and the dose tested, the pine pollen fermentation product showes no acute toxicity or genotoxicity. The no observed adverse effect level for the 28-day oral toxicity in both male and female rats are at least 2.5 g/(kg·BW). Therefore, the pine pollen fermentation product is considered to be safe for consumption.