刘 冰,李 沛,曾馨俞,刘小春,邹名洋,林 杉,赵 萍,曹建雄.三地羊肚菌菌丝体胞外多糖分离纯化及降血糖和抗氧化活性研究[J].食品安全质量检测学报,2025,16(5):86-93
三地羊肚菌菌丝体胞外多糖分离纯化及降血糖和抗氧化活性研究
Extraction and isolation of Morchella eohespera mycelium extracellular polysaccharides and its hypoglycemic and antioxidant activities
投稿时间:2024-08-19  修订日期:2025-02-17
DOI:
中文关键词:  三地羊肚菌菌丝体  胞外多糖  理化性质  降血糖活性  抗氧化活性
英文关键词:Morchella eohespera mycelium  extracellular polysaccharides  physicochemical properties  hypoglycemic activity  antioxidant activity
基金项目:甘肃省知识产权计划项目(22ZSCQ005,22ZSCQ008);兰州理工大学博士科研启动基金(062002)
作者单位
刘 冰 1. 兰州理工大学生命科学与工程学院 
李 沛 1. 兰州理工大学生命科学与工程学院 
曾馨俞 1. 兰州理工大学生命科学与工程学院 
刘小春 1. 兰州理工大学生命科学与工程学院 
邹名洋 1. 兰州理工大学生命科学与工程学院 
林 杉 1. 兰州理工大学生命科学与工程学院 
赵 萍 1. 兰州理工大学生命科学与工程学院 
曹建雄 2. 兰州理工大学理学院 
AuthorInstitution
LIU Bing 1. School of Life Science and Engineering, Lanzhou University of Technology 
LI Pei 1. School of Life Science and Engineering, Lanzhou University of Technology 
ZENG Xin-Yu 1. School of Life Science and Engineering, Lanzhou University of Technology 
LIU Xiao-Chun 1. School of Life Science and Engineering, Lanzhou University of Technology 
ZOU Ming-Yang 1. School of Life Science and Engineering, Lanzhou University of Technology 
LIN Shan 1. School of Life Science and Engineering, Lanzhou University of Technology 
ZHAO Ping 1. School of Life Science and Engineering, Lanzhou University of Technology 
CAO Jian-Xiong 2. School of Science, Lanzhou University of Technology 
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中文摘要:
      目的 以三地羊肚菌菌丝体胞外多糖(Morchella eohespera mycelium extracellular polysaccharides, MEP)为研究对象, 采用DEAE琼脂糖凝胶FF (DEAE Sepharose Fast Flow)柱层析纯化得到多糖MEP-H和MEP-N, 分别对其理化性质、体外降血糖活性和抗氧化活性进行分析。方法 通过碳水化合物含量测定、紫外扫描分析、傅里叶变换红外光谱分析和扫描电子显微镜对多糖MEP-H和MEP-N进行理化性质研究; 通过α-淀粉酶抑制率、α-葡萄糖苷酶抑制率、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除能力、2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, ABTS]阳离子自由基清除能力、还原力和超氧阴离子清除能力, 评价MEP-H和MEP-N的体外降血糖活性和抗氧化活性。结果 三地羊肚菌菌丝体胞外多糖MEP-H和MEP-N碳水化合物含量分别为(78.12±0.14)%和(77.37±0.03)%。降血糖研究表明, 当质量浓度0.75 mg/mL时, MEP-H和MEP-N对α-淀粉酶抑制率最高, 分别为(8.06±1.93%)和(11.08±1.05)%; 质量浓度0.50 mg/mL时, MEP-H和MEP-N对α-葡萄糖苷酶抑制率最高, 分别为(74.93±2.72)%和(69.48±2.97)%。抗氧化研究表明, MEP-H和MEP-N在质量浓度分别为4 mg/mL、2 mg/mL时达到最佳DPPH自由基清除活性, 清除率分别为(47.54±10.88)%和(47.16±6.91)%; 质量浓度为8 mg/mL时, MEP-H和MEP-N的最大ABTS阳离子自由基清除率分别为(8.67±0.53)%和(17.00±4.21)%, 最大还原力吸光值分别为0.13±0.004和0.17±0.008, 最大超氧阴离子清除率分别为(40.95±6.02)%和(29.87±3.18)%。结论 羊肚菌菌丝体胞外多糖MEP-H和MEP-N均具有降血糖活性和抗氧化活性, 本研究为液态发酵羊肚菌的深入研究提供了理论依据。
英文摘要:
      Objective To study the Morchella eohespera mycelium extracellular polysaccharides (MEP), purify MEP-H and MEP-N by DEAE Sepharose Fast Flow column chromatography, and analyze their physicochemical properties, hypoglycemic activities, and antioxidant activities in vitro. Methods The physicochemical properties of MEP-H and MEP-N were studied by carbohydrate content determination, analysis of ultraviolet scanning, Fourier transform infrared spectroscopy analysis, and scanning electron microscopy. The hypoglycemic activities and antioxidant activities of MEP-H and MEP-N were evaluated by α-amylase inhibition rate, α-glucosidase inhibition rate, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) cationic free radical scavenging ability, reducing power and superoxide anion scavenging ability. Results The carbohydrate content of MEP-H and MEP-N was (78.12±0.14)% and (77.37±0.03)%, respectively. Hypoglycemic studies showed that at a mass concentration of 0.75 mg/mL, MEP-H and MEP-N had the highest α-amylase inhibition rates, which were (8.06±1.93)% and (11.08±1.05)%, respectively; at a mass concentration of 0.50 mg/mL, MEP-H and MEP-N had the highest α-glucosidase inhibition rates, which were (74.93±2.72)% and (69.48±2.97)%, respectively. Antioxidant studies showed that MEP-H and MEP-N achieved the best DPPH free radical scavenging activity at mass concentrations of 4 mg/mL and 2 mg/mL, with scavenging rates of (47.54±10.88)% and (47.16±6.91)%, respectively; at a mass concentration of 8 mg/mL, the maximum ABTS cationic free radical scavenging rates of MEP-H and MEP-N were (8.67±0.53)% and (17.00±4.21)%, respectively, the maximum reducing power absorbance values were 0.13±0.004 and 0.17±0.008 respectively, and the maximum superoxide anion scavenging rates were (40.95±6.02)% and (29.87±3.18)%, respectively. Conclusion Both MEP-H and MEP-N, the extracellular polysaccharides from the mycelium of Morchella eohespera, exhibit hypoglycemic and antioxidant activities. This study provides a theoretical basis for further research on liquid fermentation of Morchella eohespera.
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