黑芝麻花水提液抗氧化活性及其黄酮类化合物分析

Antioxidant activity and flavonoids analysis of water extract of black sesame flower

中文关键词:  黑芝麻花  抗氧化  自由基  黄酮  飞行时间质谱

英文关键词:Black sesame flower  Antioxidant  Free radicals  Flavonoids  Time-of-flight mass spectrometry

中文摘要:

      目的 探究不同固液比的黑芝麻花水提液的抗氧化能力和黄酮含量，鉴定黑芝麻花中所含的黄酮种类。方法 以黑芝麻花为原料，分别测定不同固液比的黑芝麻花水提液的黄酮含量、1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazine，DPPH)自由基清除率和2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt，ABTS]阳离子自由基清除率，并对其抗氧化能力和黄酮含量进行相关性分析。同时采用超高效液相色谱-四级杆飞行时间串联质谱(ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS)对黑芝麻花的黄酮类物质组成进行定性分析。结果 随着固液比的增大，黑芝麻花水提液的DPPH自由基清除率和ABTS阳离子自由基清除率逐渐增加上升，黄酮含量也不断增高，。当固液比由1:250增大到1:100时，DPPH自由基清除率从27.20%上升到61.68%，ABTS阳离子自由基清除率从28.98%上升到68.31%，黄酮含量从0.287增加到0.631 mg芦丁/g。黄酮含量与DPPH自由基清除率、ABTS阳离子自由基清除率呈显著正相关(P＜0.01)，DPPH自由基清除率和ABTS阳离子自由基清除率呈显著正相关(P＜0.01)；并在黑芝麻花中鉴定出了85种黄酮类化合物，分别为异鼠李素-3-O-葡萄糖苷、芦丁、毛蕊花糖苷、漆黄素、山柰酚-3-O-葡萄糖醛酸苷、pratensein-7-O-glucoside、芹菜素、黄芩苷。结论 黑芝麻花水提液具有良好的抗氧化活性，不同固液比的水提液表现出不同的黄酮含量和抗氧化能力。黄酮作为黑芝麻花的生理活性成分之一，具有一定的应用价值和开发前景，本研究在黑芝麻花中鉴定到了8种黄酮类化合物，不仅丰富了芝麻花中黄酮类化合物种类，还为黑芝麻花的功能评价和开发利用提供参考。

英文摘要:

      Objective To investigate the antioxidant capacity and content of flavonoids of water extract in black sesame flower with different ratio of solid-liquid, and identify the types of flavonoids in black sesame flower. Methods Using black sesame flower as raw material, content of flavonoids, scavenging rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) cationic free radical of water extract in black sesame flower with different ratio of solid-liquid were detected, and the correlation between its antioxidant capacity and content of flavonoids was analyzed. Meanwhile, ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used to qualitatively analyze composition of flavonoids in black sesame flower. Results With the increase of the ratio of solid-liquid, the scavenging rate of DPPH free radical and ABTS cationic free radical of water extract in black sesame flower gradually increased, content of flavonoids also increased, When the ratio of solid-liquid increase from 1:250 to 1:100, the scavenging rate of DPPH free radical increased from 27.20% to 61.68%, the scavenging rate of ABTS cationic free radical increased from 28.98% to 68.31%, and the flavonoid content increased from 0.287 mg rutin/g to 0.631 mg rutin/g. There was a significant positive correlation between the scavenging rate of DPPH free radical and ABTS cationic free radical (P<0.01)， and the content of flavonoids was significantly positively correlated with the scavenging rate of DPPH free radical and ABTS cationic free radical (P<0.01). The 8 kinds of flavonoids were identified in black sesame flower, including isorhamnetin-3-O-glucoside, rutin, verbascoside, fisetin, kaempferol-3-O-glucuronside, pratensein-7-O-glucoside, apigenin, baicalin. Conclusion The water extract of black sesame flower has good antioxidant activity, and the water extract with different ratio of solid-liquid shows different the content of flavonoids and antioxidant capacity. As one of the physiological active components of black sesame flower, the flavonoid have certain application value and development prospect. In this study, 8 kinds of flavonoids are identified in black sesame flower, which not only enriched the types of flavonoids in sesame flower, but also provide a reference for the functional evaluation and development of black sesame flower.