水果蔬菜中甲拌磷残留胶体金免疫层析快速检测方法评估与分析

Evaluation and analysis of rapid colloidal gold immunochromatographic detection method for phorate residues in fruits and vegetables

中文关键词:  甲拌磷  快速检测  检出限  交叉反应

英文关键词:phorate  rapid detection  limit of detection  cross-reactivity

中文摘要:

      目的 制定食品快检快速检测方法《水果蔬菜中甲拌磷残留的快速检测 胶体金免疫层析法》的技术指标。方法 依据《中华人民共和国农业部公告 第1490号》和GB 2763—2021《食品安全国家标准 食品中农药最大残留限量》, 初步拟定适用范围和检出限, 并筛选符合要求的快速检测产品。依据《市场监管总局关于规范食品快速检测使用的意见》对快速检测产品进行评价, 初步确定该方法的性能指标和交叉反应。采用真实阳性样品测试该方法的检测应用效果, 并分析比对该方法与参比方法的一致性。随机选择5家相关专业机构进行实验间验证。结果 该方法适用于11类蔬菜(鳞茎类、芸薹属类、叶菜类等)、6类水果(柑橘类、仁果类、核果类等)中甲拌磷残留的快速检测, 检出限为0.01 mg/kg, 灵敏度≥95%、特异性≥95%、假阴性率≤5%、假阳性率≤5%; 该方法与甲拌磷砜交叉反应率≥50%, 与甲拌磷亚砜交叉反应率≥50%, 与甲胺磷、乙酰甲胺磷、水胺硫磷、甲基对硫磷、甲基硫环磷的交叉反应率均<0.1%, 与杀螟硫磷的交叉反应率≤0.1%, 与对硫磷的交叉反应率≤10%。结论 该方法可以作为新制订标准, 为甲拌磷快速检测试剂盒生产提供规范统一的技术指标参数, 应用于水果蔬菜中甲拌磷残留量的监测。

英文摘要:

      Objective To establish the technical specifications for the rapid detection method of food of Rapid detection of methyl mixed phosphorus residues in fruits and vegetables-Colloidal gold immunochromatography. Methods According to the Announcement No.1490 of the ministry of agriculture of the People’s Republic of China and the GB 2763—2021 National food safety standard-Maximum residue limits for pesticides in food, the scope of application and limits of detection were preliminarily formulated, and the rapid detection products meeting the requirements were screened. According to the Opinions of the general administration of market supervision on regulating the use of rapid detection of food, the rapid detection products were evaluated, and the performance indicators and cross-reactivity of the method were preliminatively determined. The efficacy of the method was evaluated using naturally contaminated positive samples, and its consistency with the reference method was analyzed and compared. Inter-laboratory validation was conducted with 5 randomly selected professional institutions. Results The method was found to be suitable for the rapid detection of phorate residues in 11 types of vegetables (bulbous, Brassica, and leafy varieties, etc.) and 6 types of fruits (Citrus, pome, and stone fruits, etc.), with a limit of detection of 0.01 mg/kg. Sensitivity and specificity were both≥95%, false-negative rate was≤5% and false-positive rate was≤5%. Cross-reactivity rates were≥50% with phorate sulfone and phorate sulfoxide, while cross-reactivity was<0.1% with methamidophos, acephate, isocarbophos, parathion-methyl, and phosfolan-methyl. Cross-reactivity with fenitrothion was≤0.1%, and with parathion was≤10%. Conclusion This method has been established as a new standard, providing standardized technical specifications for the production of rapid detection kits for phorate. It is suitable for monitoring phorate residue levels in fruits and vegetables.