文冠果油纳米乳液的制备及其特性表征

Preparation and characterization of the nanoemulsion of Xanthoceras sorbifolia Bunge oil

中文关键词:  文冠果油  乳清蛋白  水包油纳米乳液  粒径  稳定性

英文关键词:Xanthoceras sorbifolia Bunge oil  whey protein  Oil-in-water nanoemulsion  particle size  stability

中文摘要:

      目的 探究文冠果油纳米乳液的最佳制备工艺并进行响应面优化,并对制得的纳米乳液进行特性表征,以增强食品中生物活性物质的稳定性和生物利用度。方法 以乳清蛋白作为水相,文冠果油作为油相,在高速剪切作用下制备水包油型纳米乳液。通过单因素实验探究水相比、油相比、剪切速度、剪切时间、pH等因素对文冠果油纳米乳液的粒径及Zeta电位的影响；通过设计四因素三水平的响应面实验优化制备工艺,并测定最佳工艺条件下制备的纳米乳液的热稳定性、盐稳定性、体外消化活性和流变特性。结果 文冠果油纳米乳液的最佳制备工艺为:水相比3.5%、油相比9.8%、剪切速度15000 r/min、剪切时间3 min,在此条件下制备的文冠果油纳米乳液的平均粒径为3.57 nm,而且具有良好的稳定性和体外消化活性,表现为弹性凝胶结构。结论 本研究建立的文冠果油纳米乳液制备工艺可行且稳定性良好,拓宽文冠果油的应用领域,具有一定的理论价值和应用价值。

英文摘要:

      Objective To investigate the optimum preparation process of the emulsion of Xanthoceras sorbifolia Bunge oil and the response surface optimization, and to characterize the emulsion that enhance the stability and bioavailability of bioactive substances in food. Methods Oil in water nanoemulsion was prepared by high-speed shear with whey protein as water phase and Xanthoceras sorbifolia Bunge oil as oil phase. The effects of water phase ratio, oil phase ratio, rotate speed, rotate time and pH on the preparation of the particle size and Zeta potential of nanoemulsion of Xanthoceras sorbifolia Bunge oil were investigated by single factor experiment. A response surface experiment with 4 factors and 3 levels was designed to optimize the preparation process, and the thermal stability, salt stability, in vitro digestion activity and rheological properties of the nanoemulsion prepared under the optimal process conditions were determined. Results The optimum preparation techniques were as follows: the ratio of water phase to 3.5%, the ratio of oil phase to 9.8%, the rotate speed to 15000 r/min, and the rotate time to 3 min. Under these conditions, the average particle size of the nanoemulsion was 3.57 nm, and it had good stability and digestive activity in vitro, showing an elastic gel structure. Conclusion The nanoemulsion preparation process established in this study is feasible and stable, which can broaden the application of the Xanthoceras sorbifolia Bunge oil, and has certain theoretical and application value.