高效液相色谱法测定辣椒制品中辣椒素含量及辣度差异性分析

Determination of capsaicin content in pepper products by high performance liquid chromatography and analysis of difference in spiciness

中文关键词:  高效液相色谱法  辣椒素  显著差异性

英文关键词:high performance liquid chromatography  Capsaicin  Significant difference

中文摘要:

      建立并优化基于高效液相色谱法测定辣椒素制品中辣椒素含量的方法,并对方法进行验证。

英文摘要:

      A method for the determination of capsaicin in capsaicin products based on high performance liquid chromatography was established and optimized, and the method was verified. At the same time, the difference analysis of spiciness of different pepper products in the market was discussed. The separation was performed on an ODS column ( 250 mm × 4.6 mm, 5 μm ) with a mobile phase of methanol-water at a flow rate of 1.0 mL / min. The detection wavelength was set at 280 nm. Results : Capsaicin had a good linear relationship with the peak area in the range of 0.001-0.15mg / mL ( R2 = 0.9990 ), and the average recovery rate was 98.22 % -98.89 %. This method is relatively simple and suitable for the detection of total capsaicin in pepper products.The optimized method was used to determine the pepper products, and it was found that the spiciness of different pepper products was significantly different ( P < 0.05 ). This method has the advantages of simple operation, strong specificity, high accuracy and good repeatability. It can be used for the determination of capsaicin and has a far-reaching impact on the technical improvement of pepper products and the research results of the difference of pepper varieties.