超高效液相色谱-四极杆串联离子阱质谱法同时检测动物源性食品中26种新型抗病毒药物

Simultaneous determination of 26 kinds of antiviral drugs in animal derived foods by ultra performance liquid chromatography quadrupole tandem ion trap-mass spectrometry

中文关键词:  抗病毒药物  超高效液相色谱-四极杆串联离子阱质谱仪  动物源性食品  QuEChERS EMR-Lipid

英文关键词:Antiviral drugs  Ultra high performance liquid chromatography quadrupole tandem ion trap mass spectrometer  Food of animal origin  QuEChERS EMR-Lipid

中文摘要:

      目的:本文建立了一种应用超高效液相色谱-四极杆串联离子阱质谱仪对动物源性食品中多种抗病毒药物同时检测的方法及筛查方法。方法:样品经三氯乙酸-乙腈(20:80)溶液提取,QuEChERS EMR-Lipid净化,UPLC-MS/MS检测,MRM-IDA-EPI确证。结果:26种抗病毒药物定量限为0.1 μg/kg~5 μg/kg,相关系数均高于0.992,回收率范围为71%~97%,相对标准偏差为1.3%~10.7%。结论:采用新型通过型净化技术及四极杆串联离子阱质谱,可同时对26种抗病毒药物进行定性、定量检测；采用二级全扫描谱图与谱库检索比对,实现化合物的确证分析。

英文摘要:

      Objective:This article establishes a method and screening method for simultaneous detection of multiple antiviral drugs in animal derived foods using ultra-high performance liquid chromatography quadrupole tandem ion trap mass spectrometer. Method:The sample was extracted with a solution of trichloroacetic acid acetonitrile (20:80), purified with QuEChERS EMR Lipid, detected by UPLC-MS/MS, and confirmed by MRM-IDA-EPI. Results:The quantitative limit of antiviral drugs is 0.1 μ g/kg~5 μ g/kg, with correlation coefficients higher than 0.992. The recovery rate range is 71%~97%, and the relative standard deviation is 1.3%~10.7%. Conclusion:By adopting a new type of purification technology and quadrupole tandem ion trap mass spectrometer, qualitative and quantitative detection of 26 antiviral drugs can be carried out simultaneously; By using a second level full scan spectrogram and comparing it with the spectral library retrieval, the confirmation analysis of compounds can be achieved.