基于3种不同培养基的食品中真菌培养组学方法研究

Research on fungal culturomics methods in food based on 3 kinds of different culture media

中文关键词:  食品  真菌  培养组学  分离培养

英文关键词:fungi  culturomics  isolation and culture

中文摘要:

      目的 通过对比3种真菌培养基的性能, 优化真菌培养组学技术, 建立食品中真菌培养组学解决方案。方法 利用食品检测常用的真菌培养基马铃薯葡萄糖琼脂(potato dextrose agar, PDA)、孟加拉红琼脂(rose Bengal agar)和1种新型Pan-Fungal真菌培养基(Pan-Fungal medium, PF)检测24份食品样本和2份能力验证样品。采用基质辅助激光解吸电离-飞行时间质谱法(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS)对培养出的真菌进行分离鉴定。结果 酵母相真菌在3种培养基上均能良好生长, 菌落生长速度、菌落形态、大小及菌落数基本一致。丝状真菌在3种培养基上能正常生长, 但生长的速度和菌落形态均有明显的差异。24个样品共鉴定出12种真菌, 其中酵母相真菌2种, 丝状真菌10种。结论 PF真菌培养基具有良好的准确性和稳定性, 且培养出的菌落呈自限性生长, 有利于单菌落的分离鉴定, 可作为食品中常用真菌培养基的补充, 用于食品中真菌的培养组学研究。

英文摘要:

      Objective To establish a solution for fungal culturomics in food by comparing the performance of 3 fungal culture media and optimising fungal culturomics techniques. Methods The 24 food samples and 2 proficiency test samples were tested using commonly used fungal culture media for food testing, including potato dextrose agar (PDA), rose Bengal agar, and a novel Pan Fungi medium (PF). The cultured fungi were isolated and identified using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results Yeast phase fungi could grow well on all 3 kinds of media, and the colony growth rate, colony morphology size and colony numbers had no significant difference. Filamentous fungi were able to grow well on the 3 kinds of medias, but growth rate and colony morphology were significantly different. There were 12 kinds of fungal species identified in the 24 samples, including 2 kinds of yeast phase fungi and 10 kinds of filamentous fungi. Conclusion With excellent accuracy and stability and self-limited growth of cultured colonies, PF fungal medium is favourable for isolation and identification of single colonies, and can be used as a supplement to the commonly used fungal medium for fungal culturomics studies in food.