超高效液相色谱-串联质谱法快速检测速冻调制食品中7种甜味剂与防腐剂

Rapid determination of 7 kinds of sweeteners and preservatives in quick-frozen prepared food by ultra performance liquid

中文关键词:  甜味剂  防腐剂  超高效液相色谱-串联质谱法  速冻调制食品

英文关键词:sweeteners  preservatives  ultra performance liquid chromatogram-tandem mass spectrometry  quick-frozen prepared food

中文摘要:

      目的 基于超高效液相色谱-串联质谱法（ultra performance liquid chromatography-tandem mass spectroscopy, UPLC-MS/MS）建立速冻调制食品中7种甜味剂与防腐剂的高通量快速检测方法。方法 采用Phenomenex Kinetex F5色谱柱(50 mm×2.1 mm, 2.6 μm)，以0.05%甲酸-10 mmol/L乙酸铵和乙腈进行梯度洗脱，电喷雾离子源(electrospray ionization, ESI)负离子模式、多反应监测(multiplereaction monitoring, MRM)模式进行测定。结果 在0.5~5.0 mg/L质量浓度范围内,目标物的线性关系良好,相关系数均大于0.99, 方法检出限(S/N=3)为10~50 μg/kg; 在不同添加质量浓度(1.0、2.0、10.0 mg/kg)下, 其回收率为81.2%~106.5%、相对标准偏差均≤10%。结论 该方法具有操作简便、快速、准确、灵敏等优点，可满足速冻调制食品中7种甜味剂与防腐剂的定性定量分析，为速冻调制食品中防腐剂与甜味剂的快速筛查和风险分析奠定基础。

英文摘要:

      ABSTRACT：Objective To establish an analytical method for rapid detection of seven sweeteners and preservatives in quick-frozen prepared foods by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods A Phenomenex Kinetex F5 column (50 mm×2.1 mm, 2.6 μm) was used, and the gradient elution was performed with 0.05% formic acid-10 mmol/L ammonium acetate and acetonitrile. The electrospray ionization (ESI) negative ion mode and multiple reaction monitoring (MRM) mode were used for detection. Results The target substance exhibits a good linear relationship within the concentration range of 0.5-5.0 mg/L, as indicated by correlation coefficients greater than 0.99. The detection limit (S/N=3) of the method is 10-50 μg/kg; At different concentrations (1.0,2.0,10.0 mg/kg), the recovery rate ranged from 81.2% to 106.5%, and the relative standard deviation was ≤10%. Conclusion This method is straightforward to use, fast, highly accurate, and sensitive, and is capable of performing qualitative and quantitative analysis of seven types of sweeteners and preservatives in quick-frozen prepared foods. It provides a basis for rapid screening and risk assessment of preservatives and sweeteners in these foods.