高效液相色谱-串联质谱法测定食品中7种红曲色素

Determination of 7 kinds of Monascus pigments in food by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  红曲色素  液相色谱串联质谱  食品添加剂  PRiME HLB

英文关键词::monascus pigments  ultra performance liquid chromatography-triple quadrupole mass spectrometry  food additives  PRiME HLB

中文摘要:

      目的 采用通过式固相萃取进行前处理，建立了高效液相色谱-串联质谱法测定食品中7种红曲色素。方法 样品经过乙腈提取，Oasis PRiME HLB固相萃取柱（200 mg，6 cc）净化，Waters ACQUITY UPLC BEH C18 (100 mm×2.1 mm，1.7 μm)色谱柱分离，采用高效液相色谱串联质谱仪检测，基质匹配外标法定量。考察了豆制品、糕点、酸奶、腐乳、熟肉、饮料以及腊肠7种基质中红曲色素回收率。结果 7种基质中潘红胺、红曲红胺、红曲素、红斑素、红曲黄素、红曲玉红素、红曲红素的加标回收率分别为73.9%~112.1%，70.1%~92.6%，72.4%~103.4%，73.8%~93.5%，73.4%~113.3%，71.6%~105.7%，75.0%~112.1%；7种色素的相对标准偏差为0.2%~15.7%；红曲黄素的检出限为3.0 μg/kg，其余6种色素的检出限为1.0 μg/kg。结论 该方法前处理简单快速、灵敏度高、准确度和精密度良好，采用基质匹配的标准曲线定量，适用于检测不同基质中7种红曲色素。

英文摘要:

      To established a method for the determination of Monascus pigments in foods by pass-through solid phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS). Methods Sample was extracted by acetonitrile, purified by Oasis PRiME HLB (6 cc, 200 mg, Waters), Waters ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7μm) column was used for separation; ultra-performance liquid chromatography-triple quadrupole mass spectrometry was used for determination, and matrix matching standard method was used for quantitative analysis. Bean products, flour product, yogurt, fermented bean curd, coloring meat products and fruit juice were selected as substrates to verify the recoveries of 7 monascus pigments. Results In the validation, the recoveries of different concentrations of Rubropunctamine, Monascus amine, Monascin, Rubropunctation, Ankaflavin, Monascorubrin and Monascus red were 73.9%- 112.1% ,70.1%-92.6%, 72.4%~103.4%, 73.8%~93.5%, 73.4%~113.3%, 71.6%~105.7%, 75.0%~112.1% respectively; the relative standard deviation of 7 monascus pigments was 0.2%~15.7% .The detection limits of ankaflavin was 3.0 μg·kg-1，the rest 6 monascus pigments detection limit was 1.0 μg·kg-1.Conclusion The method is simple and rapid，with high sensitivity, good accuracy and precision, which can test 7 monascus pigments in different food, used matrix matching standard curve.