瓜子等不同食品中二氧化硫假阳性的快速验证及实际应用分析

Rapid verification of sulfur dioxide false positives in different foods such as melon seeds and analysis of practical applications

中文关键词:  二氧化硫、假阳性、快速验证、酸碱滴定、离子色谱

英文关键词:sulfur dioxide  false positive  rapid verification  acid-base titration  ion chromatography

中文摘要:

      目的 探究快速验证瓜子等不同食品中二氧化硫假阳性及实际应用分析。方法 对快速验证法采取空白实验、检出限和定量限验证、加标回收、精密度实验、阳性样品不同方法比对等方式进行方法学验证, 以确保该方法的可行性。同时以瓜子等食品为研究对象, 用快速验证法对GB 5009.34—2022《食品安全国家标准 食品中二氧化硫的测定》中酸碱滴定法检出的阳性样品进行验证, 并与GB 5009.34—2022离子色谱法的验证结果进行比对, 最后对上述假阳性的可疑性成因进行了初步探讨分析。结果 快速验证法能快速、有效地验证GB 5009.34—2022酸碱滴定法的假阳性现象, 验证结果与GB 5009.34—2022离子色谱法一致。研究发现GB 5009.34—2022酸碱滴定法检测瓜子、黑木耳干出现假阳性的概率较高, 部分辣椒、八角等香辛料、酱腌菜、黄花菜干等蔬菜干制品、代用茶、其他炒货类等也会出现假阳性现象。结论 GB 5009.34—2022酸碱滴定法检测瓜子等食品二氧化硫会出现不同程度的微量检出现象, 当结果不确定时, 可用本研究的快速验证法进行快速验证, 该法验证结果稳定可靠, 较GB 5009.34—2022其他验证方法验证效率更高、更快。

英文摘要:

      Objective To explore the rapid verification of sulfur dioxide false positive in different foods such as melon seeds and practical application analysis. Methods The method of rapid verification was validated by blank experiment, limit of detection and limit of quantification verification, label recovery, precision experiment and comparison of different methods of positive samples to ensure the feasibility of the method. At the same time, melon seeds and other foods were taken as objects, and the positive samples detected by acid-base titration in GB 5009.34—2022 National standards for food safety-Determination of sulfur dioxide were verified by rapid verification method, and compared with the verification results of GB 5009.34-2022 ion chromatography. Finally, the suspicious causes of the above false positives were discussed and analyzed. Results The rapid verification method could quickly and effectively verify the false positive phenomenon of acid-base titration by GB 5009.34—2022, and the verification results were consistent with that of ion chromatography by GB 5009.34—2022. The study found that GB 5009.34—2022 acid-base titration method had a higher probability of false positive in the detection of melon seeds and black dried fungus, and some spices such as chili pepper, star anise, dried vegetable products such as pickled vegetables, dried daylily, substitute tea, and other fried goods would also appear false positive phenomenon. Conclusion GB 5009.34—2022 acid-base titration method will detect sulfur dioxide in foods such as melon seeds to varying degrees. When the results are uncertain, the rapid verification method in this paper can be used for rapid verification. The verification results of this method are stable and reliable, and the verification efficiency is higher and faster than other verification methods in GB 5009.34—2022.