桑黄多糖提取工艺优化、结构表征及抗氧化活性研究

Optimization of extraction process, structural characterization and antioxidant activity of Sanghuangporus sanghuang polysaccharides

中文关键词:  桑黄多糖  热水浸提  工艺优化  结构表征  抗氧化活性

英文关键词:Sanghuangporus sanghuang polysaccharide  hot water extraction  process optimization  structural characterization  antioxidant activity

中文摘要:

      目的 以临清桑黄为研究材料, 优化桑黄多糖(Sanghuangporus sanghuang polysaccharide-hot water extraction, SSP-W)的制备工艺并研究其结构特征和抗氧化活性。方法 以SSP-W提取得率为指标, 采用单因素和响应面实验对制备工艺进行优化, 采用高效阴离子交换色谱-脉冲安培检测法(high performance anion exchange chromatography-pulsed amperometric detection, HPAEC-PAD)、傅里叶变换红外光谱(Fourier transform infrared spectroscopy, FT-IR)、扫描电镜(scanning electron microscopy, SEM)和X射线衍射(X-ray diffraction, XRD)对其结构进行初步表征。结果 最佳制备工艺为: 提取时间2.4 h、提取温度95℃、液料比27:1 (mL/g), 在此条件下, SSP-W提取得率为2.34%。其结构特征为: SSP-W以吡喃环为基本骨架, 由岩藻糖、氨基葡萄糖、葡萄糖和甘露糖4种单糖组成。扫描电镜结果表明, SSP-W形状不规则, 有明显的孔洞。X-衍射结果表明, SSP-W以结晶态聚合物和非结晶态聚合物的形式共存。抗氧化实验表明, SSP-W具有较强的抗氧化活性, 其羟自由基和2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, ABTS]阳离子自由基的半最大效应浓度(concentration for 50% of maximal effect, EC50)值分别为488 μg/mL和75 μg/mL, 当质量浓度为250.0 μg/mL时, ABTS阳离子自由基清除率达到96.27%。结论 SSP-W是一种具有抗氧化潜力的活性物质。本研究结果将为临清桑黄多糖的精深加工和高值化利用提供科学依据。

英文摘要:

      Objective To optimize the preparation process of Sanghuangporus sanghuang polysaccharide-hot water extraction (SSP-W) using hot water extraction from Linqing, and study its structural characteristics and antioxidant activity. Methods The preparation process was optimized by one-way and response surface tests using the SSP-W extraction rate as an indicator. High performance anion exchange chromatography-pulsed amperometric detection (HPAEC-PAD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used for the preliminary structural characterization. Results The optimal extraction conditions were: Extraction time 2.4 h, extraction temperature 95℃, liquid-feed ratio 27:1 (mL/g), and the extraction rate was 2.34%. SSP-W had a pyran ring as the basic skeleton, which consists of fucose, glucosamine, glucose and mannose. SEM showed that the shape of SSP-W was irregular, with obvious pores. X-diffraction showed that SSP-W coexisted in the form of crystalline polymers and amorphous polymers. The antioxidant activity results showed that SSP-W had strong antioxidant activity, the concentration for 50% of maximal effect (EC50) of SSP-W hydroxyl radical scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) cationic free radical scavenging were 488 μg/mL and 75 μg/mL, the ABTS cationic free radical scavenging rate reached 96.27% at a concentration of 250.0 μg/mL. Conclusion SSP-W is an active substance with antioxidant potential, the results provide scientific basis for the deep processing and high value utilization of Linqing Sanghuangporus sanghuang polysaccharide.