枯草芽孢杆菌抗菌脂肽的重组表达及应用研究进展

Research progress on encapsulation systems to improve the bioavailability of plant polyphenols

中文关键词:  枯草芽孢杆菌  抗菌脂肽  重组表达

英文关键词:Bacillus subtilis  antimicrobial lipopeptide  recombinant expression

中文摘要:

      为了解决对人类健康和粮食安全构成重大风险的抗生素耐药性威胁, 开发新型抗菌药物势在必行。枯草芽孢杆菌所产的抗菌脂肽不易使细菌产生广泛的耐药性且无任何副作用, 被认为是最具潜力的抗菌剂之一。但是, 枯草芽孢杆菌抗菌脂肽生产成本高和生产效率低的问题严重限制了其在食品、医药、环境等工农业领域的应用, 因此提高抗菌脂肽产量显得尤为重要。近几十年来, 随着生物技术的快速发展, 利用基因工程和优化发酵策略来生产高产量的抗菌脂肽可以克服上述问题。本文从枯草芽孢杆菌所产脂肽的结构和功能特性出发, 主要从高产抗菌脂肽技术、构效关系以及应用进行综述, 特别是基因工程方法提高脂肽产量的研究进展, 为枯草芽孢杆菌高产抗菌脂肽的进一步研究提供参考。

英文摘要:

      To address the antibiotic resistance threats that pose significant risks to human health and food security, it is imperative to develop new antibiotics. The antimicrobial lipopeptides produced by Bacillus subtilis are not prone to widespread resistance of bacteria without any side effects and are considered to be one of the most potential antibacterial agents. However, the problem of high production cost and low production efficiency of antimicrobial lipopeptides from Bacillus subtilis seriously limits its application in food, medicine, environment and other industrial and agricultural fields, so it is particularly important to improve the yield of antibacterial lipopeptides. With the rapid development of biotechnology in recent decades, the above issues can be overcome using genetic engineering and to optimize fermentation strategies. Based on the structure and functional characteristics of lipopeptides produced by Bacillus subtilis, this paper mainly focused on the high-yield lipopeptide technology, structure-activity relationship and application, especially the research progress of genetic engineering method to improve lipopeptide yield, so as to provide reference for further research of high-yield antimicrobial lipopeptides in Bacillus subtilis.