基于确定性筛选设计优化柑橘皮抗氧化活性成分提取工艺

Optimization of extracting craft for antioxidant active ingredient in citrus peel based on definitive screening design

中文关键词:  确定性筛选设计  设计空间  抗氧化活性成分  柑橘皮  提取

英文关键词:definitive screening design  design space  antioxidant active ingredients  citrus peel  extraction

中文摘要:

      目的 基于确定性筛选设计优化柑橘皮抗氧化活性成分提取工艺研究。方法 首先采用确定性筛选设计考察料液比、碱用量、醇体积分数、提取次数、提取温度和提取时间6个因素对提取效果的影响。然后, 代表性抗氧化活性成分橙皮苷、芸香柚皮苷、圣草次苷的峰面积之和作为评价指标, 6个因素作为变量因素, 通过多元回归算法建立定量模型。基于模拟实验测定误差法计算获得关键因素与评价指标之间的设计空间。结果 提取过程中, 抗氧化活性成分的关键影响因素有3个, 分别为提取次数、提取温度和料液比, 并成功验证。该3个因素推荐的操作空间是提取次数为1.5~2.9, 提取温度为51~60℃, 料液比为30.2:1~35.0:1 (mL:g)。结论 本研究优选了提取工艺涉及的6个参数对抗氧化活性成分提取的影响, 推荐的操作空间可使代表性抗氧化活性成分的峰面积达标概率达到0.96以上, 为工业化提取柑橘皮抗氧化活性成分提供了参考。

英文摘要:

      Objective To optimization of the extraction process of antioxidant active components from citrus peel based on deterministic screening design. Methods Firstly, a definitive screening design was adopted to investigate the effects of 6 factors, including solid-liquid ratio, alkali amount, ethanol concentration, extracting frequency, temperature and time. Then, the sum of peak areas of representative antioxidant active ingredients hesperidin, narirutin, and eriocitrin were chosen as evaluation indicators, and 6 factors were used as variable factors. Hence, a quantitative calibration analysis model was established through multiple regression algorithm. Finally, designing space between key factors and evaluation indicator was calculated based on the simulation experiment measurement error method. Results During the extraction process, there were 3 key influencing factors of antioxidant active ingredients, namely extracting frequency, extracting temperature and solid-liquid ratio, which had been successfully validated. The recommended operating space was the frequency of extraction from 1.5–2.9, extraction temperature in the range of 51–60℃, and a solid liquid ratio of 30.2:1–35.0:1. Conclusion This study selected 6 parameters involved in the extraction process to investigate their impact on the extraction of antioxidant active ingredients. The recommended operating space can ensure that the peak area of representative antioxidant active ingredients meets the standard probability of 0.96 or above, providing a reference for industrial extraction of antioxidant active ingredients from citrus peel.