超高效液相色谱-串联质谱法测定动物源性食品中多种肾上腺素类药物残留

Determination of multiple adrenaline drug residues in animal derived food by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  肾上腺素  动物源性食品  快速分散固相萃取  液相色谱-串联质谱

英文关键词:adrenaline  animal derived food  QuEChERS  ultra performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立一种超高效液相色谱-串联质谱法同时测定动物源性食品中肾上腺素类、拟肾上腺素类、肾上腺素代谢物等10种肾上腺素类药的方法。方法 本研究采用乙腈提取, 分散固相萃取法(QuEChERS）净化, Shim-pack GIST C18色谱柱, 甲醇和含0.1%甲酸的5 mmol/L乙酸铵水溶液作为流动相, 超高效液相色谱-串联质谱法确证检测, 外标法定量。结果 10种肾上腺素类物质在一定线性范围内关系良好, 相关系数均高于0.9946, 方法定量限为0.1~2.0 μg/kg, 回收率为75.6%~107.5%, 相对标准偏差为1.1%~8.7%。结论 该方法具有检测药物种类多、最低检测限低、稳定性好、简便快捷、节省试剂等优点, 为更好的控制市场上动物源性食品的品质提供的技术支撑。

英文摘要:

      Objective To establish a method for simultaneous determination of 10 kinds of adrenaline related drugs composed of adrenaline, pseudoadrenaline, and adrenaline metabolites in animal derived food using ultra performance liquid chromatography-tandem mass spectrometry. Methods This study used acetonitrile extraction, dispersion solid-phase extraction (QuEChERS) purification, Shim pack GIST C18 chromatographic column, methanol and 5 mmol/L ammonium acetate aqueous solution containing 0.1% formic acid as mobile phases, ultra performance liquid chromatography tandem mass spectrometry to confirm detection, and external standard method for quantification. Results The relationship between 10 kinds of adrenaline like substances was good within a certain linear range, with correlation coefficients higher than 0.9946. The limit of quantitative of the method was 0.1–2.0 μg/kg, the recovery rates wwere 75.6%–107.5%, and the relative standard deviations were 1.1%–8.7%. Conclusion This method has the advantages of detecting multiple types of drugs, low detection limit, good stability, simplicity and speed, and saving reagents, providing technical support for better controlling the quality of animal derived foods in the market.