超高效液相色谱-串联质谱法测定凉茶中10种硝基咪唑类物质

Determination of 10 kinds of nitroimidazole substances in herbal tea by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  凉茶  超高效液相色谱-串联质谱法  硝基咪唑类物质

英文关键词:herbal tea  ultra performance liquid chromatography-tandem mass spectrometry  nitroimidazole substances

中文摘要:

      目的 建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)测定凉茶中硝基咪唑类物质含量的方法。方法 试样经50%甲醇水溶液超声提取, 采用T3色谱柱(2.1 mm×100 mm, 1.6 μm)分离, 建立UPLC-MS/MS测定二甲硝咪唑、羟基二甲硝咪唑、异丙硝唑、羟基甲硝唑、羟基异丙硝唑、氯甲硝咪唑、洛硝哒唑、替硝唑、奥硝唑、甲硝唑10种成分含量的方法。结果 UPLC-MS/MS检测10种成分平均回收率为64.9%~118.0%, 精密度为2.0%~13.0%, 在一定浓度范围内, 线性关系均大于0.99, 检出限为0.025~0.125 mg/kg, 定量限为0.050~0.250 mg/kg。结论 该方法操作简单、准确性高、稳定性好, 可用于凉茶中10种硝基咪唑类成分的定量分析。

英文摘要:

      Objective To establish a method for the determination of nitroimidazole compounds in herbal tea by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was extracted by ultrasound with 50% methanol aqueous solution and separated using a T3 (2.1 mm × 100 mm, 1.6 μm) chromatographic column. A method was established for the determination of the content of 10 kinds of components, including dimetridazole, 1-methyl-5-nitro-2-hydroxymethylimidazole, ipronidazole, metronidazole-OH, Ipronidazole-hydroxy, 5-chloro-1-methyl-4-nitroimid-azole, ronidazole, tindazole, ornidazole, and metronidazole, using UPLC-MS/MS. Results The average recovery rates of 10 kinds of components detected by UPLC-MS/MS ranged from 64.9%-118.0%, with precision ranging from 2.0%-13.0%. Within a certain concentration range, the linear relationship was greater than 0.99. The detection limit was 0.025–0.125 mg/kg, and the limits of quantification were 0.050–0.500 mg/kg. Conclusion This method is easy to operate, has high accuracy, and good stability, and can be used for quantitative analysis of 10 kinds of nitroimidazole components in herbal tea.