酸化甘油-超声辅助提取杨梅花青素工艺优化及其抗氧化活性研究

Optimization of acid glycerol-ultrasonic assisted extraction process for anthocyanins from Myrica rubra Sieb. & Zucc. and study on its antioxidant activity

中文关键词:  杨梅  花青素  酸化甘油-超声辅助  响应面优化  抗氧化

英文关键词:Myrica rubra Sieb. & Zucc.  anthocyanins  acidified glycerol-ultrasound assisted extraction  response surface methodology  antioxidant activity

中文摘要:

      目的 通过响应面实验确定酸化甘油-超声辅助提取杨梅花青素最佳提取参数, 并探究其体外抗氧化活性。方法 研究了酸化甘油浓度、提取时间、超声功率和提取温度4个单因素对杨梅花青素得率的影响, 并优化花青素提取工艺参数。此外, 考察了最佳提取工艺下杨梅花青素对3种氧化自由基的清除率和还原力等指标, 评估了其抗氧化活性。结果 优化后的酸化甘油-超声辅助提取杨梅花青素的工艺条件为: 酸化甘油浓度16%、提取时间42 min、超声功率204 W、提取温度46℃, 此条件下杨梅花青素的得率为(6.915±0.027) mg CGE/g DW, 接近预测值(6.961 mg CGE/g DW)。提取的杨梅花青素质量浓度为100 μg/mL时, 对超氧阴离子自由基、羟基自由基、1,1-二苯基-2-三硝基苯肼自由基清除率分别为59.35%、46.77%和61.65%。该浓度下, 杨梅花青素的还原力为维生素C的39.84%。结论 本研究成功地应用响应面法优化了酸化甘油-超声辅助提取杨梅花青素的提取参数, 并且提取物展现出良好的抗氧化活性。

英文摘要:

      Objective To optimize the extraction parameters for anthocyanins from Myrica rubra Sieb. & Zucc. using acidified glycerol-ultrasound assisted extraction through response surface methodology, and explore its in vitro antioxidant activity. Methods The effects of 4 single factors, including acidified glycerol concentration, extraction time, ultrasound power, and extraction temperature, on the extraction yield of anthocyanins from Myrica rubra Sieb. & Zucc. were studied, then the extraction process parameters were optimized. In addition, the scavenging rates of 3 types of oxidative free radicals and reducing power of anthocyanin under the optimal extraction process were evaluated. Results The optimized extraction conditions for anthocyanins of Myrica rubra Sieb. & Zucc. were as follows: Acidified glycerol concentration of 16%, extraction time of 42 min, ultrasound power of 204 W, extraction temperature of 46℃. Under these conditions, the anthocyanin yield was (6.915±0.027) mg CGE/g DW, which closed to the predicted value (6.961 mg CGE/g DW). At the concentration of 100 μg/mL, the scavenging rates of anthocyanins on superoxide anion radical, hydroxyl radical, 1,1-diphenyl-2-trinitrobenzene radical were 59.35%, 46.77% and 61.65%, respectively. At this concentration, the reducing power of anthocyanins from Myrica rubra Sieb. & Zucc. was 39.84% of vitamin C. Conclusion This study successfully applies response surface methodology to optimize the extraction parameters of acidified glycerol-ultrasound assisted extraction of anthocyanins from Myrica rubra Sieb. & Zucc., and the extract exhibits good antioxidant activity.