基于超微消解结合电感耦合等离子体质谱法的核桃产地溯源

Traceability of the origin of walnut based on ultra-microlysis combined with inductively coupled plasma mass spectrometry

中文关键词:  电感耦合等离子体质谱法  矿物质元素  主成分分析  特征元素  聚类分析

英文关键词:inductively coupled plasma mass spectrometry  mineral elements  main component analysis  feature elements  cluster analysis

中文摘要:

      目的 探究6种产地来源核桃中20种矿物质元素与种植区域的相关性, 实现产地溯源。方法 样品经超微消解仪处理后, 利用电感耦合等离子体质谱仪测定20种矿物质元素含量, 对数据进行主成分分析, 筛选出特征元素, 并对特征元素与种植区域的相关性进行系统聚类分析。结果 核桃样品中20种矿物质元素检测方法的线性关系良好, 相关系数大于0.99, 检出限为0~24.23 μg/L, 该方法的精密度、稳定性的相对标准偏差均小于7.4%, 样品加标回收率为80.4%~119.3%、相对标准偏差为2.1%~7.9% (n=3)。6种产地来源核桃样品中, 常量元素由高到低是K、Mg、Ca, 大部分样品微量元素含量由高到低为Mn、Fe、Zn、Cu。主成分分析确定Na、Sr、Mn、Cu、Rb、Ni、Zn、Al和Cs 9种元素为甘肃徽县、陕西商南县、四川盐边县、云南漾濞县、新疆喀什及阿克苏地区共36批核桃中矿物质元素的特征成分。结论 主成分分析和特征元素分析发现不同产区核桃元素含量的差异较大, 本研究可以应用在核桃产地鉴定中, 为核桃的产地溯源体系建设提供参考和技术支持。

英文摘要:

      Objective To explore the correlation between 20 kinds of mineral elements and planting areas of the source of 6 kinds of origin sources to achieve traceability of origin. Methods After the samples were processed by the ultrafine digester, the contents of 20 kinds of mineral elements were determined by the inductively coupled plasma mass spectrometer. Principal component analysis was performed on the data, and the characteristic elements were screened out. The correlation between characteristic elements and planting areas was analyzed by systematic clustering. Results The linear relationship of 20 kinds of mineral elements in walnut samples were good, the correlation coefficients were greater than 0.99, and the limits of detection were 0–24.23 μg/L. The relative standard deviations of precision and stability of the method were all less than 7.4%. The recoveries were 80.4%–119.3% and the relative standard deviations were 2.1%–7.9% (n=3). Among the 6 kinds of walnut samples from different origins, the major elements from high to low are K, Mg, and Ca, while the majority of the samples had trace element content from high to low of Mn, Fe, Zn, and Cu. Principal component analysis identified 9 kinds of elements, namely Na, Sr, Mn, Cu, Rb, Ni, Zn, Al, and Cs, as characteristic components of mineral elements in 36 batches of walnuts from Huixian County, Gansu Province, Shangnan County, Shaanxi Province, Yanbian County, Sichuan Province, Yangbi County, Yunnan Province, Xinjiang Kashgar and Aksu. Conclusion The main component analysis and characteristic element analysis found that the content of walnut elements in different production areas is large. This study can be applied in the identification of walnuts, providing reference and technical support for the construction of the traceability system of walnuts.