菊花和三七对乙醇氧化损伤小鼠的保护作用

Protective effects of Chrysanthemum and Panax notoginseng on mice damaged by ethanol oxidation

中文关键词:  菊花  三七  乙醇氧化损伤  抗氧化

英文关键词:Chrysanthemum  Panax notoginseng  ethanol oxidative damage  antioxidation

中文摘要:

      目的 探究菊花提取物和三七提取物对乙醇氧化损伤小鼠的保护作用。方法 实验小鼠随机分为空白对照组, 模型对照组, 低、中、高菊花提取物组, 低、中、高三七提取物组, 菊花三七混合组。对小鼠进行灌胃30 d后, 除空白对照组外均灌胃50%乙醇, 建立乙醇氧化损伤小鼠模型。检测各组小鼠肝脏和脑组织细胞中的抗氧化物质以及血清中的肝损伤检测指标, 观察小鼠皮肤和肝组织的病理变化, 研究菊花提取物和三七提取物对乙醇氧化损伤小鼠的作用。结果 由机体抗氧化的指标可见, 三七提取物和菊花提取物及其混合物能够有效降低小鼠肝脏及脑组织中的丙二醛、蛋白质羰基含量以及丙氨酸转氨酶、天冬氨酸转氨酶、总胆固醇和甘油三酯在血清中的水平, 提高肝脏和脑组织中超氧化物歧化酶、谷胱甘肽和谷胱甘肽过氧化物酶的含量。小鼠肝组织和皮肤的苏木精-伊红染色结果表明, 与模型组相比, 各剂量组小鼠皮肤因乙醇氧化损伤所致组织病理改变均有所减轻。各剂量组小鼠的皮肤与肝组织因乙醇氧化损伤所致组织病理改变均有所减轻。急性毒性实验显示, 菊花提取物、三七提取物对小鼠没有毒害作用。结论 菊花提取物、三七的提取物以及菊花三七混合物对小鼠乙醇氧化损伤模型有保护作用。

英文摘要:

      Objective To investigate the effects of Chrysanthemum extract and Panax notoginseng extract on ethanol-induced oxidative damage in mice. Methods The experimental mice were randomly divided into a blank control group, model control group, low, medium and high chrysanthemum extract groups, low, medium and high Panax notoginseng extract groups, and a mixed group of Panax notoginseng and chrysanthemum extract. After 30 days of continuous gavage, all mice except the blank control group were gavaged with 50% ethanol to establish a mouse model of ethanol oxidative damage. The antioxidant substance levels in liver and brain tissue cells as well as liver injury indicators in serum were measured. Additionally, pathological changes in skin and liver tissue were observed to study the effects of chrysanthemum extract and Panax notoginseng extract on ethanol-induced oxidative injury in mice. Results The results of the body’s antioxidant indicators showed that the extracts of Panax notoginseng and Chrysanthemum can effectively reduce the levels of malondialdehyde, protein carbonyl, alanine aminotransferase, aspartate aminotransferase, total cholesterol, and triglyceride in the liver and brain tissues of mice, and increase the content of superoxide dismutase, glutathione, and glutathione peroxidase in the liver and brain tissues. The hematoxylin-eosin staining results of the skin and liver tissues of mice showed that the pathological changes in the skin and liver tissues caused by ethanol oxidation damage in the mice of all dosage groups were alleviated. The acute toxicity experiment showed that Chrysanthemum extract and Panax notoginseng extract had no toxic effects on mice. Conclusion Chrysanthemum extract, Panax notoginseng extract and the mixture of chrysanthemum and Panax notoginseng exhibited protective effects against ethanol-induced oxidative damage in a mouse model.