基于荧光法快速判定巴氏杀菌乳和超高温灭菌乳

Rapid determination of pasteurized milk and ultra high temperature milk by fluorescence method

中文关键词:  巴氏杀菌乳  超高温灭菌乳  荧光强度  综合热损伤指数  鉴别

英文关键词:pasteurized milk  ultra high temperature milk  fluorescence intensity  fluorescence of advanced Maillard products and soluble tryptophan index  identification

中文摘要:

      目的 基于荧光法快速简便地鉴别巴氏杀菌乳和超高温(ultra high temperature, UHT)灭菌乳。方法 基于美拉德产物荧光值的不同, 采用直接荧光法结合综合热损伤(fluorescence of advanced Maillard products and soluble tryptophan, FAST)指数快速鉴定巴氏杀菌乳和UHT灭菌乳。结果 巴氏杀菌乳和UHT灭菌乳由于热处理强度不同, 二者的荧光值存在显著差异(P<0.01), 巴氏杀菌乳荧光值在(13215±236)~(15359±156)之间、UHT灭菌乳荧光值范围为(15788±200)~(20440±270)。与巴氏杀菌乳相比, UHT灭菌乳直接测定荧光强度和FAST指数明显升高且差异显著(P<0.01)。在商品巴氏杀菌乳和UHT灭菌乳结果的基础上, 利用自制产品对荧光法进行了验证。结论 直接荧光测定法和FAST指数能够快速有效区分和识别巴氏杀菌乳和UHT灭菌乳, 该技术对产品热处理强度评价及质量监管具有一定指导意义。

英文摘要:

      Objective To distinguish pasteurized milk and ultra high temperature (UHT) sterilized milk quickly and easily by fluorescence methods. Methods Based on the different fluorescence values of Maillard products, direct fluorescence assay combined with fluorescence of advanced Maillard products and soluble tryptophan (FAST) index was used to identify pasteurized milk and UHT sterilized milk rapidly. Results The fluorescence values of pasteurized milk and UHT milk were significantly different due to the different heat treatment intensity (P<0.01). The fluorescence values of pasteurized milk ranged from (13215±236) to (15359±156), and the fluorescence values of UHT milk ranged from (15788±200) to (20440±270). Compared with pasteurized milk, the fluorescence intensity and FAST index measured directly by UHT sterilized milk were significantly increased and the difference was significant(P<0.01). In addition, on the basis of the results of commercial pasteurized milk and UHT pasteurized milk, the fluorescence method was verified by using lab-made products. Conclusion The direct fluorescence assay and FAST index can distinguish between pasteurized milk and UHT pasteurized milk rapidly and effectively. This technology has a certain guiding significance for heat treatment strength evaluation and quality supervision of products.