气相色谱法测定婴幼儿配方乳粉中的1,3-二油酸-2-棕榈酸甘油三酯

Determination of 1,3-dioleic-2-palmitate triglyceride in infant formula milk powder by gas chromatography

中文关键词:  1,3-二油酸-2-棕榈酸甘油三酯  配方乳粉  气相色谱法

英文关键词:1,3-dioleic-2-palmitate triglyceride  formula milk powder  gas chromatography

中文摘要:

      目的 建立气相色谱法测定婴幼儿配方乳粉中1,3-二油酸-2-棕榈酸甘油三酯(1,3-dioleyl-2- palmitoyl-glycerol, OPO)的方法。方法 样品通过碱式水解处理, 提取脂肪后, 采用甘油三酯专用气相色谱柱进行分离, 并使用氢火焰离子化检测器进行检测, 结果采用外标法定量。结果 OPO在0.02~1.00 mg/mL质量浓度范围内具有良好的线性关系, 相关系数(r2)为0.999。检出限与定量限分别为0.119 g/100 g和0.664 g/100 g, 回收率为99.1%~100.1%, 相对标准偏差小于5%。应用此方法对我国市售的36批次OPO强化婴幼儿配方乳粉进行检测, OPO含量为2.39~4.89 g/100 g, 占标签标示值的60.7%~102.1%。这主要因为本方法检测的是OPO含量, 而标签标示的是碳数为C52的甘油三酯的量。结论 该方法成功实现了OPO与其他碳数为C52、饱和度不同的甘油三酯的气相色谱法基线分离, 可对OPO单体进行精确的定性和定量分析, 适用于婴幼儿配方乳粉中OPO的检测。

英文摘要:

      Objective To establish a method for determination of 1,3-dioleic-2-palmitate triglyceride (OPO) in infant formula milk powder by gas chromatography. Methods The samples were treated by alkaline hydrolysis, the fat was extracted, the triglyceride was separated by gas chromatography column, and the hydrogen flame ionization detector was used for detection. The results were quantified by external standard method. Results OPO had a good linear relationship in the mass concentration range of 0.02–1.00 mg/mL, and the correlation coefficient (r2) was 0.999. The limits of detection and quantification were 0.119 g/100 g and 0.664 g/100 g, respectively. The recoveries were 99.1%-100.1%, and the relative standard deviations were less than 5%. This method was used to detect 36 batches of OPO-fortified infant formula powder sold in China, and the OPO content was 2.39–4.89 g/100 g, accounting for 60.7%–102.1% of the labeled value. This was mainly because this method detects the OPO content, while the label indicated the amount of triglycerides with a carbon number of C52. Conclusion The method successfully realizes the separation of OPO from other triglycerides with carbon number of C52 and different saturation by gas chromatography, and can be used for the accurate qualitative and quantitative analysis of OPO monomer, which is suitable for the detection of OPO in infant formula milk powder.