超高效液相色谱-四极杆-飞行时间-高分辨质谱法分析6种李果实中的代谢物差异性

Analysis of differences of metabolites in 6 kinds of plum fruit by ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry

中文关键词:  李果实  超高效液相色谱-四极杆-飞行时间-高分辨质谱  代谢物  差异性分析

英文关键词:plum fruit  ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry  metabolite  difference analysis

中文摘要:

      目的 采用超高效液相色谱-四极杆-飞行时间高分辨质谱法分析6种李果实中的代谢物差异。方法 使用天然产物高分辨二级谱库、代谢物高分辨二级谱库和相关数据库等对不同李果实样品中的代谢产物进行数据分析, 利用峰面积归一化法计算各化学成分的相对含量, 采用主成分分析方法、偏最小二乘法判别分析和方差分析等方法, 分析不同李果实样品中代谢产物的差异。结果 共分析鉴定了130个化学成分, 其中包括28个黄酮类、25个脂肪酸、18个萜烯类、11个核苷酸、10个酚酸、9个香豆素类、8个含氮化合物、6个酚类以及15个其他类化合物; 在所鉴定的130个化合物中, 有123个化合物对各组李果实均值间的差异具有统计学意义(P<0.05); 6组不同品种李果实的数据间具备很好的区分度, 贡献最大的化合物共有32个。结论 6个品种李果实存在相同种类的代谢产物, 各代谢产物在不同品种李果实中的相对含量存在显著差异性, 为李果品种选育、区分以及产地溯源等提供参考。

英文摘要:

      Objective To analyze the difference of metabolites in 6 kinds of plum fruit by ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry. Methods High resolution secondary spectrum of natural products, high resolution secondary spectrum of metabolites and related databases were used to analyze the data of metabolites in different plum fruit samples. Peak area normalization method was used to calculate the relative content of each chemical component. Principal component analysis, partial least square discriminant analysis and variance analysis were used to analyze the differences of metabolites in different plum fruit samples. Results A total of 130 compounds were identified, including 28 flavonoids, 25 fatty acids, 18 terpenes, 11 nucleotides, 10 phenolic acids, 9 coumarins, 8 nitrogen compounds, 6 phenols and 15 other compounds. Among the 130 compounds identified, 123 compounds showed statistically significant differences between the mean values of plum fruit groups (P<0.05). There was a good degree of differentiation among the 6 groups of different varieties of plum fruit, and a total of 32 compounds with the greatest contribution. Conclusion The same metabolites are found in 6 varieties of plum fruit, and the relative content of metabolites in different kinds of plum fruit are significantly different, which provides reference for breeding, distinguishing and origin tracing of plum fruit varieties.