椰枣多糖缓解功能性便秘的作用研究

Studying the impact of Phoenix dactylifera L. fruit polysaccharides on relieving functional constipation

中文关键词:  椰枣  多糖  排便试验  小肠运动试验  水通道蛋白

英文关键词:Phoenix dactylifera L.  polysaccharides  defecation test  small bowel motility test  aquaporins

中文摘要:

      目的 在动物水平探究椰枣多糖的通便活性。方法 采用洛哌丁胺诱导的功能性便秘小鼠模型评价椰枣多糖。将小鼠随机分为空白对照组、模型对照组、阳性对照组和低、中、高剂量椰枣多糖组; 连续处理7 d后, 通过排便实验和小肠运动实验来评价椰枣多糖的通便效果; 采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction, qPCR)检测结肠组织中水通道蛋白相关基因的表达。结果 排便实验结果表明, 椰枣多糖能够显著缩短便秘小鼠首粒排黑便时间, 明显增加6 h内的粪便粒数、粪便湿重以及粪便含水率; 小肠运动实验结果表明, 椰枣多糖显著增加便秘小鼠小肠墨汁推进率, 且呈现出一定的剂量依赖性; qPCR结果表明, 椰枣多糖显著下调AQP3的基因表达、上调AQP9的基因表达。结论 椰枣多糖能够显著改善洛哌丁胺引起的小鼠便秘症状, 且通便效果温和、无不良副作用, 可能通过调节水通道蛋白基因的表达从而增加便秘小鼠粪便含水量。

英文摘要:

      Objective To study the laxative activity of Phoenix dactylifera fruit polysaccharide at animal level. Methods The functional constipation mice model induced by loperamide was used to evaluate the laxative effect of Phoenix dactylifera fruit polysaccharide. The mice were divided into different groups: A blank control group, a model control group, a positive control group, and groups receiving low, medium, and high doses of Phoenix dactylifera fruit polysaccharide. After 7 days of consistent treatment, we assessed the laxative effect of Phoenix dactylifera fruit polysaccharide by conducting defecation and small intestinal motility tests. Additionally, we utilized quantitative real-time polymerase chain reaction (qPCR) to detect the expression of aquaporin-related genes in colon tissue. Results The defecation test results indicated that Phoenix dactylifera fruit polysaccharide could significantly reduce the time for the first appearance of black stool in constipated mice, as well as notably increase the number of feces, the wet weight of stool, and the water content of stool within 6 hours. The small intestinal motility test results indicated that Phoenix dactylifera fruit polysaccharide significantly boosted the ink propulsion rate in the small intestine of constipated mice in a dose-dependent manner. The qPCR results showed that the Phoenix dactylifera fruit polysaccharide significantly decreased the gene expression of AQP3 and increased the gene expression of AQP9. Conclusion Phoenix dactylifera fruit polysaccharide can significantly improve constipation symptoms in mice induced by loperamide. They have a mild laxative effect with no adverse side effects, potentially increasing the water content of feces in constipated mice by regulating the expression of aquaporin genes.