高效液相色谱法同时检测小麦粉中6种非法添加剂

Determination of 6 kinds of illegal additives in wheat flour by high performance liquid chromatography

中文关键词:  小麦粉  非法添加剂  高效液相色谱法

英文关键词:wheat flour  illegal additives  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定小麦粉中硫脲、曲酸、间苯二酚、苯甲羟肟酸、三聚硫氰酸三钠盐和噻二唑6种非法添加剂。方法 样品经70%乙腈水溶液提取, 采用Thermo Hypersil-Gold C18液相色谱柱, 使用0.1%磷酸水溶液-甲醇作为流动相进行等度洗脱, 二极管阵列检测器检测。结果 6种非法添加剂在0.5~20.0 μg/mL范围内均展现出良好的线性关系(r2>0.999)。方法检出限均为0.2 μg/g; 平均回收率为62.9%~100.1%, 相对标准偏差为1.16%~2.65% (n=6)。结论 该方法检测效率高、稳定性好, 具有良好的应用前景。

英文摘要:

      Objective To establish a method for the determination of 6 kinds of illegal additives in wheat flours by high performance liquid chromatography, including thiourea, kojic acid, resorcinol, benzohydroxamic acid, trisodium thiocyanate, thiadiazole. Methods The sample was extracted with a 70% acetonitrile aqueous solution and subjected to Thermo Hypersil-Gold C18 liquid chromatography column. The 0.1% phosphoric acid aqueous solution methanol was used as the mobile phase for isocratic elution and detected by a diode array detector. Results Six kinds of illegal additives showed good linear relationships (r2>0.999) in the range of 0.5–20.0 μg/mL. The limits of detection of the method were all 0.2 μg/g; the average recoveries were 62.9%–100.1%, and the relative standard deviations were 1.16%–2.65% (n=6). Conclusion This method has high detection efficiency, good stability, and good application prospects.