红曲色素的抗氧化活性及对HepG2氧化损伤的保护作用研究

Study on antioxidant activity of monascus pigments and its protective effects on oxidative damage of HepG2

中文关键词:  红曲色素  自由基清除  HepG2细胞  抗氧化  

英文关键词:monascus pigments  free radical scavenging  HepG2 cell  antioxidation

中文摘要:

      目的 研究纯化后的红曲色素(monascus pigments, MPs)抗氧化活性及对HepG2细胞氧化损伤的修复作用。方法 以红曲米粉为原料, 对其含有的MPs进行提取、纯化和鉴定。通过测定MPs的总抗氧化能力、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基和2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, ABTS]阳离子自由基清除率评估了其抗氧化活性。同时, 选用0.20 mmol/L的H2O2建立氧化应激模型, 探索不同浓度MPs对细胞氧化损伤的保护机制。结果 纯化后的MPs含有4种MPs单体, 分别为安卡红曲黄素、红曲玉红素、红曲素和潘红胺。MPs的总抗氧化能力(total antioxidant capacity, T-AOC)、DPPH自由基和ABTS阳离子自由基清除率与其浓度呈正相关。用不同浓度的MPs预处理后, 与MC组相比, 较低浓度(10 μg/mL)的MPs能显著提高细胞的存活率, 但较高浓度的MPs (20 μg/mL)显著降低了细胞的存活率, 这可能是H2O2与MPs协同作用导致的。随着MPs浓度增加, 细胞中活性氧累积量逐渐减少。当MPs质量浓度为20 μg/mL时, 过氧化氢酶和超氧化物歧化酶活性分别从14.75 U/mL±0.04 U/mL、3.87 U/mL±0.09 U/mL提升至15.25 U/mL±0.05 U/mL、8.29 U/mL±0.68 U/mL。结论 MPs有较强的自由基清除能力, 对H2O2诱导的氧化损伤有保护作用, 这可能是与MPs提高了抗氧化酶活性, 降低了细胞内ROS累积量有关。

英文摘要:

      Objective To investigate the antioxidant activity and regulatory mechanism of post-purified monascus pigments (MPs). Methods The MPs contained in monascus rice flour was extracted, purified and identified. The antioxidant activity of MPs was evaluated by measuring its total antioxidant capacity, radical scavenging rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and cationic radical of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS). At the same time, 0.20 mmol/L H2O2 was selected to establish an oxidative stress model, and the protective mechanism of different concentrations of MPs on oxidative damage of cells was explored. Results The purified MPs contains 4 kinds of MPs monomers. The total antioxidant capacity (T-AOC), DPPH radical and ABTS cation radical scavenging rates of MPs were positively correlated with their concentrations. After pretreatment with different concentrations of MPs, it was found that the lower concentration (10 μg/mL) of MPs could significantly improve the cell survival rate, but the high concentration of MPs (20 μg/mL) significantly reduced the cell survival rate, which may be caused by the synergistic effect of H2O2 and MPs. With the increase of MPs concentration, the accumulation of reactive oxygen species in cells gradually decreased. When the mass concentration of MPs was 20 μg/mL, the activities of catalase and superoxide dismutase increased from 14.75 U/mL±0.04 U/mL and 3.87 U/mL±0.09 U/mL to 15.25 U/mL±0.05 U/mL and 8.29 U/mL±0.68 U/mL, respectively. Conclusion MPs has a strong ability to scavenge free radicals, and has a protective effect on H2O2-induced oxidative damage, which may be related to the increase of antioxidant enzyme activity and the decrease of intracellular ROS accumulation.