甘草中20种无机元素的测定及对有害元素的健康风险评估

Determination of 20 kinds of inorganic elements in Glycyrrhizae radix et rhizoma and their health risk assessment

中文关键词:  甘草  电感耦合等离子体质谱；原子荧光光谱法  健康风险评估

英文关键词:Glycyrrhizae radix et rhizoma  inductively coupled plasma-mass spectroscopy  atomic fluorescence spectrometry  health risk assessment

中文摘要:

      目的 测定甘草中无机元素含量, 并对重金属及有害元素进行污染分析及健康风险评估。方法 采用电感耦合等离子体质谱法及原子荧光光谱法测定甘草中的无机元素含量及数据分析。通过计算样品中Pb、Cd、As、Hg和Cu 5种重金属及有害元素的单向污染指数和综合污染指数对其进行安全性评价, 通过计算每日最大可耐受摄入量(maximum tolerable daily intake, EDI)、靶标危害系数(target hazard quotients, THQ)和致癌风险(carcinogenic risk, CR), 对甘草中5种重金属元素和有害元素进行健康风险评估, 计算出甘草中各重金属的推荐重金属限量值(maximum residue limit, MRL)。结果 得到的各无机元素在各自范围内线性关系良好(0.9969≤R2≤0.9996), 样品稳定性、重复性、加标回收率及仪器精密度良好; Cr、Mn、Ni、Cu、Rb、Sr和Ba元素含量较高, 并且各样品间各元素含量具有一致性和差异性; 主成分分析中22个样品得到5个主成分, 累计方差贡献率达84.308%, 确定Ga、V、Cs、Se、Co、As、U、Ni、Cd和Hg是甘草的特征无机元素; 相关性分析结果与主成分分析结果基本一致; 健康风险评估结果显示甘草样品均对暴露人群无明显健康危害, 其致癌风险也可忽略不计。结论 本研究所建立的方法可有效进行甘草无机元素的测定及来源判别, 并为甘草健康风险评估提供了科学依据。

英文摘要:

      Objective To determine the content of inorganic elements in Glycyrrhizae radix et rhizoma, and carry out pollution analysis and health risk assessment on heavy metals and harmful elements. Methods The content of inorganic elements in Glycyrrhizae radix et rhizoma was determined by inductively coupled plasma-mass spectrometry and atomic fluorescence spectrometry. The safety was evaluated by calculating the one-way pollution index and comprehensive pollution index of Pb, Cd, As, Hg and Cu of 5 kinds of heavy metals and harmful elements in the samples. By calculating the maximum tolerable daily intake (EDI), target hazard quotients (THQ) and carcinogenic risk (CR), the health risks of 5 kinds of heavy metal elements and harmful elements in Glycyrrhizae radix et rhizoma were assessed, and the recommended maximum residue limit (MRL) of each heavy metal in Glycyrrhizae radix et rhizoma was calculated. Results The obtained inorganic elements had good linearity in their respective ranges (0.9969≤R2≤0.9996), sample stability, repeatability, spiked recovery and instrument precision were good. The content of Cr, Mn, Ni, Cu, Rb, Sr and Ba elements was high, and the content of each element was consistent and different among the samples. In the principal component analysis, 5 principal components were obtained from 22 samples, and the cumulative variance contribution rate was 84.308%. It was determined that Ga, V, Cs, Se, Co, As, U, Ni, Cd and Hg were the characteristic inorganic elements of Glycyrrhizae radix et rhizoma. The results of correlation analysis were consistent with those of principal component analysis. The results of health risk assessment showed that Glycyrrhizae radix et rhizoma samples had no obvious health hazard to the exposed population, and the cancer risk was negligible. Conclusion The method can effectively determine the inorganic elements and identify the source of Glycyrrhizae radix et rhizoma, and provide a scientific basis for the health risk assessment of Glycyrrhizae radix et rhizoma.