食源性金黄色葡萄球菌生物膜形成调控与生物防控策略研究进展

Advance on regulation of biofilm formation and bio-prevention and control of foodborne Staphylococcus aureus

中文关键词:  食源性致病菌  金黄色葡萄球菌  生物膜  基因调控因子  生物防控

英文关键词:foodborne pathogens  Staphylococcus aureus  biofilm  gene regulatory factors  bio-prevention and control

中文摘要:

      生物膜是很多食源性致病菌应对各种极端环境、杀菌理化因子以及维持菌体内环境稳定的重要基质屏障。数据显示, 有超过80%的细菌感染由生物膜引起, 尤以金黄色葡萄球菌感染多见。食源性金黄色葡萄球菌是引发细菌性食物中毒和食品安全事故的重要风险源, 特别是多重耐药菌株。金黄色葡萄球菌的耐药性、致病性和免疫逃逸与生物膜复杂的三维结构有重大关系。由于生物膜结构基因和调控因子结构相对保守, 现已成为金黄色葡萄球菌生物防控新的重要的效应靶点。本文从多糖细胞间黏附素、胞外DNA和生物膜形成相关蛋白等角度阐明了生物膜形成机制, 从群体感应系统(如Agr系统和LuxS/AI-2群体感应系统)、全局性调控因子(如附属调节因子Sar和转录因子SigB)及双组分信号转导系统(如SrrAB系统、SaeRS系统、ArlRS系统、LytRS系统和WalKR系统)等角度系统阐述了生物膜形成调控机制。最后, 从抗菌肽(蛋白)、植物源化合物、生物酶、抗菌药物等角度提出新的防控策略, 以期为食源性金黄色葡萄球菌的生物防控提供指导。

英文摘要:

      Biofilm is an important matrix barrier for many foodborne pathogens to respond to various extreme environments, bactericidal physicochemical factors, and maintain the internal stable environment of bacteria. Research datum shows that over 80% of bacterial infections are caused by biofilms, especially for Staphylococcus aureus (S. aureus). Foodborne S. aureus is an important risk source for bacterial food poisoning and food safety accidents, especially for multidrug resistant strains. The drug resistance, pathogenicity, and immune escaping of S. aureus are closely related to the complex three-dimensional structure of biofilm. Biofilm has become a new and important target for the prevention and control of S. aureus because of its relatively conservative structural genes and regulatory genes. This article elucidated that the biofilm formation mechanism from the perspectives of polysaccharide intercellular adhesion (PIA), extracellular DNA (eDNA) and biofilm-forming related proteins, and the biofilm regulatory mechanism from the following perspectives, including: Quorum sensing system (QS), such as Agr quorum sensing system and LuxS/AI-2 quorum sensing system; global regulatory factors, such as accessory regulatory factor Sar and transcription factor SigB; two-component systems (TCS), such as SrrAB TCS, SaeRS TCS, ArlRS TCS, LytRS TCS and WalKR TCS. Finally, some new strategies for the bio-prevention and control of foodborne S. aureus were put forward from the perspectives of antibacterial peptide (protein), plant products-derived antibiofilm agents, enzymes and antibiotics, etc., and hope to provide guidance for the biological prevention and control of S. aureus.