光甘草定包合物对中波紫外线诱导小鼠皮肤光损伤的保护作用

Protection of glabridin inclusion on middlewave ultraviolet radiation induced skin photo damage in mice

中文关键词:  环糊精包合  光甘草定  中波紫外线  光损伤

英文关键词:cyclodextrin inclusion  glabridin  middlewave ultraviolet radiation  photo damage

中文摘要:

      目的 以环糊精(cyclodextrin, CD)为载体制备光甘草定(glabridin, GL)包合物, 探讨光甘草定环糊精包 合物(GL/CD)对中波紫外线(middlewave ultraviolet radiation, UVB)诱导的小鼠皮肤光损伤的保护作用。方法 制 备 GL/CD, 进行包封率和透射电子显微镜(transmission electron microscope, TEM)表征分析, 通过 UVB 照射小鼠 皮肤光损伤模型, 在小鼠背部皮肤涂抹不同浓度的 GL 及 GL/CD, 通过测试经皮水分散失(trans-epidermal water loss, TEWL)、皮肤组织生化指标、免疫组化染色研究其对光损伤后皮肤屏障保护的效果。结果 GL/CD 的包封 率为 84.21%±1.36%, 平均粒径为(533.1±42.8) nm, Zeta 电位为(-30.24±1.54) mV, 表明包合物在水体系中分散为 纳米颗粒且稳定性良好。TEM 结果显示 GL/CD 分散在水体系后主要呈现为球形胶束状态。小鼠皮肤黑色素染 色结果显示GL/CD对黑色素生成的抑制效果优于GL。TEWL测试结果显示GL/CD显著降低小鼠经皮失水率, 且 效果比 GL 好。苏木精-伊红染色结果显示 GL 经 CD 包合之后能显著增强其抗炎作用, 降低 UVB 照射引起的表 皮增厚和炎症浸润。酶联免疫吸附实验结果显示 GL 经 CD 包合之后能显著提升抗氧化酶包括超氧化物歧化酶 和过氧化氢酶的生物合成, 降低活性氧及促炎细胞因子包括白细胞介素 1、白细胞介素 6 和肿瘤坏死因子 α 在体 内的表达水平。结论 GL 经过 CD 的包合, 有效提升了其在 UVB 诱导的皮肤损伤方面的保护作用。

英文摘要:

      Objective To synthesize glabridin (GL) inclusion complexes utilizing cyclodextrin (CD) as a delivery system, and elucidate the protective effects of the glabridin-cyclodextrin inclusion complexes (GL/CD) against middlewave ultraviolet radiation (UVB)-induced photo damage in murine skin. Methods The GL/CD complexes were formulated and subsequently characterized using encapsulation efficiency assays and transmission electron microscopy (TEM). A UVB irradiation-induced photo damage model in murine skin was constructed. Varying concentrations of GL and GL/CD were applied topically to the dorsal skin of mice. Protective effects on the skin barrier post UVB-induced damage were evaluated through measurements of trans-epidermal water loss (TEWL), biochemical indices of skin tissue, and immunohistochemical staining. Results The encapsulation efficiency of GL/CD was recorded as 84.21%±1.36%, with an average particle size of (533.1±42.8) nm and a Zeta potential of (-30.24±1.54) mV, indicating that the complexes dispersed in aqueous systems as nanoparticles and exhibited favorable stability. TEM images revealed that GL/CD primarily displayed as spherically micellar formations in water systems. Analysis of melanin staining in the mouse skin indicated that GL/CD more effectively inhibited melanogenesis compared to GL alone. TEWL assessments illustrated that GL/CD significantly decreased the rate of transepidermal water loss in mice, outperforming GL alone. Hematoxylin & Eosin staining indicated that the anti-inflammatory action of GL was notably enhanced post CD-encapsulation, significantly reducing the epidermal thickening and inflammatory infiltration caused by UVB irradiation. Enzyme linked immunosorbent assay results showed that post CD-encapsulation, GL notably elevated the biosynthesis of antioxidative enzymes, including superoxide dismutase and catalase, reduced reactive oxygen species, and lowered the expression levels of pro-inflammatory cytokines such as interleukin-1, interleukin-6, and tumor necrosis factor-alpha. Conclusion The encapsulation of GL with CD improves its protective effects against UVB-induced skin damage.