灰树花多糖的促进合成及其对酒精性胃损伤的缓解作用

Facilitated synthesis of Grifola frondosa polysaccharides and their palliative effect on alcoholic gastric injury

中文关键词:  灰树花  鱼腥草  多糖  慢性胃损伤  高通量

英文关键词:Grifola frondosa  Houttuynia cordata Thunb  polysaccharides  chronic stomach injury  high throughput

中文摘要:

      探究鱼腥草(Houttuynia cordata Thunb, HCT)醇提物对灰树花多糖(Grifola frondosa polysaccharide, GFP)产量的影响及对慢性酒精性胃组织损伤的缓解作用。方法 基于HCT醇提物与GF发酵制得灰树花多糖(命名为HCTGFP), 将108只KM雄性小鼠随机分为9组, 除空白组外, 各处理组每周二、四、六给药1 h后以5 mL/kg剂量灌胃50%食品级乙醇, 连续给药4周; 测定小鼠胃组织中氧化应激指标、抗炎标记物、炎症因子含量及其基因表达; 测定小鼠血清中炎症因子含量并观察小鼠胃组织病理变化; 采用16sRNA高通量测序法检测小鼠肠道菌群的变化。结果 HCT提高了37.6%胞外多糖产量(P<0.01), 且产生的HCTGFP能够有效增加超氧化物歧化酶活性、谷胱甘肽含量、降低丙二醛含量, 提高机体抗氧化水平; 有效抑制环氧合酶-2、白细胞介素-1β、白细胞介素-6和一氧化氮合酶基因的表达, 缓解体内炎症反应。在门水平上, 与模型组相比, HCTGFPH显著回调了F/B比值(P<0.05), 显著增加了拟杆菌门丰度(P<0.05)以及显著降低了厚壁菌门、脱硫菌门与变形菌门的相对丰度(P<0.05); 在属水平上, 摄入HCTGFP回调了拟普雷沃氏菌属(Alloprevotella)的相对丰度, 抑制了毛螺菌科属(Lachnospiraceae_NK4A136_group)、脱硫弧菌属(Desulfovibrio)的相对丰度, 有效调节小鼠肠道菌群稳态。结论 HCT能够显著提高GFP产量以及提升其调控小鼠胃组织氧化应激、炎症因子基因表达、肠道微生物菌群的效能, 对慢性酒精性胃损伤起到更好的保护作用。

英文摘要:

      Objective To investigate the effects of the alcoholic extract of Houttuynia cordata Thunb (HCT) on the yield of Grifola frondosa polysaccharide (GFP) and its utility to alleviate chronic alcoholic gastric tissue damage. Methods The HCT alcohol extract was added to the GF fermentation system to obtain Grifola frondosa polysaccharide (named HCTGFP). The 108 KM male mice were randomly divided into nine groups, and each group was given the corresponding drug and alcohol for 4 consecutive weeks, except for the blank group. Additionally, food-grade ethanol with 50% alcohol content was administered at a dose of 5 mL/kg on Tuesdays, Thursdays, and Saturdays of every week, one hour after the end of the drug administration. The oxidation indices, anti-inflammatory markers, inflammatory factors and their gene expression of mice were measured in the gastric tissues of the mice, serum levels of inflammatory factors were measured and pathological changes in the gastric tissues of the mice were observed, and finally the changes in the intestinal flora of the mice were detected using the 16sRNA high-throughput sequencing method. Results HCT was able to significantly increase exopolysaccharide production by 37.6% (P<0.01), and the newly generated HCTGFP was able to effectively increase superoxide dismutase activity, glutathione content, and reduce malondialdehyde content, resulting in higher body’s antioxidant levels; it was also able to effectively inhibit the expression of cyclooxygenase-2, interleukin-1beta, interleukin-6, and nitric oxide synthase genes, thereby alleviating the inflammatory response. At the phylum level, compared to the model group, the F/B ratio of the HCTGFPH group rebounded significantly (P<0.05). Furthermore, HCTGFPH significantly increased the abundance of the Bacteroidota (P<0.05) while decreasing the relative abundance of the Firmicutes, Desulfovibrio, and Proteobacteria (P<0.05). At the genus level, it also elevated the relative abundance of the Alloprevotella and suppressed the relative abundance of Lachnospiraceae_NK4A136_group and Desulfovibrio. This effectively regulated the stable state of the intestinal flora in mice. Conclusion HCT can significantly increase the yield of GFP and enhance its efficacy in regulating oxidative stress, inflammatory factor gene expression and intestinal microflora in mouse gastric tissue, which providing better protection against chronic alcoholic gastric injury.