食源性甲型肝炎病毒快速检测技术研究进展

Research progress of rapid detection technology for foodborne hepatitis A virus

中文关键词:  甲型肝炎病毒  快速检测  分子生物学  免疫学

英文关键词:hepatitis A virus  rapid detection  molecular biology  immunology

中文摘要:

      病毒在食源性疾病的爆发中起着至关重要的作用, 其中, 甲型肝炎病毒(hepatitis A virus, HAV)感染后能够致人衰弱, 并引发急性肝衰竭。由于其传染迅速, 影响范围广, HAV污染已经给消费者带来了严重的健康风险, 且在全球范围内造成了大规模的食源性疫情和经济损失。因此, 早期快速准确的检测HAV对于食品安全和疫情暴发的溯源至关重要, 以便及时确定并召回受污染的食品, 防止感染的进一步发生。传统的HAV检测方法由于检出率不高以及病毒在细胞内的培养周期长等问题, 往往耗时费力, 无法快速有效地对其进行检测。本文对目前报道较多的HAV快速检测技术进行了阐述和梳理, 对各项技术的检出限、检测时间以及优缺点进行了比较, 并对HAV快速检测技术研究进行了展望, 为后续HAV的快速检测研究提供依据, 也有助于进一步探讨与开发HAV快速检测的新技术, 以有效防止HAV的传播与危害。

英文摘要:

      Viruses play a crucial role in the outbreaks of foodborne disease, among of which, hepatitis A virus (HAV) infection can cause debilitation and acute liver failure. Due to its rapid transmission and wide range of impacts, HAV contamination has posed serious health risks to consumers and caused large-scale foodborne outbreaks and economic losses around the world. Therefore, early, rapid and accurate detection of HAV is essential for food safety and traceability of outbreaks, so that contaminated food can be identified and recalled in time to prevent further infections. Because of the low detection rates and the long incubation period of the virus in cells, HAV detection methods are often time-consuming and laborious, which make it difficult to quickly and effectively detect for it. This article described and summarized the currently reported HAV rapid detection techniques, comparing the limits of detection, detection times, as well as their advantages and disadvantages, and prospected the research on HAV rapid detection technologies, which is excepted to provide a basis for further research on rapid detection of HAV, and also contribute to the exploration and development of new technologies for rapid detection of HAV, so as to prevent the spread and harm of HAV.