表没食子儿茶素没食子酸酯与L-茶氨酸对乙醇脱氢酶和乙醛脱氢酶活性的体外协同作用

Synergistic effect of epigallocatechin gallate and L-theanine on alcohol dehydrogenase and acet aldehyde dehydrogenase in vitro

中文关键词:  表没食子儿茶素没食子酸酯  L-茶氨酸  乙醇脱氢酶  乙醛脱氢酶  协同作用

英文关键词:epigallocatechin gallate  L-theanine  alcohol dehydrogenase  aldehyde dehydrogenase  synergistic effect

中文摘要:

      目的 探究表没食子儿茶素没食子酸酯(epigallocatechin gallate，EGCG)与L-茶氨酸对乙醇脱氢酶(alcohol dehydrogenase，ADH)和乙醛脱氢酶(acetaldehyde dehydrogenase，ALDH)活性的体外协同作用。方法 设置不同浓度及组分，以0 μg/mL实验组为对照，先后进行单因素实验和最优组合验证实验，通过对ADH、ALDH活性的检测，确认EGCG、L-茶氨酸不同剂量的解酒效果并探究其最佳配比。结果 单因素实验中，样品质量浓度为200、300 μg/mL实验组相较于0 μg/mL浓度组，随着EGCG和L-茶氨酸的添加，ADH和ALDH活性显著升高(P<0.05)；最优组合验证实验中，11组不同配比组合物对ADH和ALDH活性均具有促进作用，且协同组比单因素组激活效果要更好。其中，当配比组合物为7:3时，ADH、ALDH的活性最高。结论 EGCG、L-茶氨酸解酒组合物最佳配比为7:3时ADH与ALDH活性最高，研究结果为EGCG、L-茶氨酸组合物用于抗醉酒药物研发提供科学依据。

英文摘要:

      Objective To explore the synergistic effect of epigallocatechin gallate (EGCG) and L-theaninein on alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in vitro. Methods Different concentrations and compositions were set, with the experimental group at 0 μg/mL serving as the control. Single-factor experiments and combination verification experiments were conducted. Through the detection of ADH and ALDH activities, the sobering effects of EGCG and L-theanine were confirmed, and their optimal ratio was explored. Results In the single-factor experiment, compared to the 0 μg/mL concentration group, the experimental group with a sample mass concentration of 200, 300 μg/mL showed a significant increase in ADH and ALDH activities with the addition of EGCG and L-theanine (P<0.05). In the optimal combination verification experiment, all 11 different ratio combinations exhibited a promoting effect on both ADH and ALDH activities, with the synergistic group showing a better activation effect compared to the single-factor groups. Specifically, when the ratio of the combination was 7:3, the activities of ADH and ALDH were the highest. Conclusion The optimal ratio of EGCG to L-theanine for the anti-intoxication effect is 7:3, resulting in the highest activities of ADH and ALDH. The findings provide scientific evidence for the development of drugs for anti-intoxication using EGCG and L-theanine combination.