聚集诱导发光微球免疫层析试纸条定量检测水产品中孔雀石绿

Aggregation-induced luminescent microsphere based immunochromatographic assay for the quantitative detection of malachite green in aquatic products

中文关键词:  聚集诱导发光荧光微球  孔雀石绿  免疫层析方法

英文关键词:aggregation-induced luminescence fluorescent microspheres  malachite green  immunochromatographic assay

中文摘要:

      目的 建立一种用于定量检测水产品中孔雀石绿的高灵敏免疫层析检测新方法。方法 以聚集诱导发光荧光微球为标记探针, 制备荧光免疫层析试纸条, 通过T/C比值法构建定量检测孔雀石绿的定量标准曲线, 并实现水产品中孔雀石绿的高灵敏、定量检测。结果 本方法定量检测水产品中孔雀石绿具有较高的检测灵敏度, 其50%抑制率达到0.17 μg/kg, 最低检出限为0.05 μg/kg。样本加标0.25、0.50、1.00 μg/kg的平均回收率介于116.01%~122.76%, 变异系数介于8.66%~11.71% (n=10), 表明所建立的荧光免疫层析方法定量检测水产品中孔雀石绿具有较好的准确性、精密度。通过检测30份孔雀石绿阴性以及12份阳性的水产样本, 结果表明所建立的荧光免疫层析方法无假阳性, 且检测孔雀石绿含量与液相色谱-串联质谱法具有良好的一致性(线性相关系数为0.9695)。结论 本研究以聚集诱导发光荧光微球为新型标记探针, 建立了一种高灵敏检测水产品中孔雀石绿的定量方法, 为水产品中孔雀石绿的筛查检测提供了技术支撑。

英文摘要:

      Objective To establish a new highly sensitive immunochromatographic assay for quantitative detection of malachite green in aquatic products. Methods Fluorescent immunochromatographic test strips were prepared by using aggregation-induced luminescence fluorescent microspheres as labeled probes, and the quantitative standard curve for quantitative detection of malachite green was constructed by T/C ratio method, and the highly sensitive and quantitative detection of malachite green in aquatic products was realized. Results The quantitative detection of malachite green in aquatic products by this method had high detection sensitivity, its 50% inhibition rate reaches 0.17 μg/kg, and the minimum limit of detection was 0.05 μg/kg. The average recoveries of 0.25, 0.50 and 1.00 μg/kg ranged from 116.01% to 122.76%, and the coefficient of variation ranged from 8.66% to 11.71% (n=10), indicating that the established fluorescence immunochromatographic assay for quantitative detection of malachite green in aquatic products had good accuracy and precision. By detecting 30 samples of aquatic products with malachite green negative and 12 samples with malachite green positive, the results showed that the established fluorescence immunochromatographic assay had no false positive, and the detection of malachite green content had good consistency with liquid chromatography-tandem mass spectrometry (linear correlation coefficient was 0.9695). Conclusion In this study, a highly sensitive quantitative method for the detection of malachite green in aquatic products is established by using aggregation-induced luminescence fluorescent microspheres as new labeling probes, which provide technical support for the screening and detection of malachite green in aquatic products.