王伟伟,陈 义,杨丽娟,江和源.基于非靶向代谢组学比较手工和机制信阳毛尖的代谢物差异[J].食品安全质量检测学报,2024,15(2):207-217
基于非靶向代谢组学比较手工和机制信阳毛尖的代谢物差异
Comparison of metabolite differences between hand-made and machine-made Xinyang Maojian based on non-targeted metabolomics
投稿时间:2023-12-13  修订日期:2024-01-18
DOI:
中文关键词:  信阳毛尖  手工  机制  代谢组学  差异化合物
英文关键词:Xinyang Maojian  hand-made  machine-made  metabolomics  differential compound
基金项目:浙江省科技计划项目(2022C02033-3),河南省豫南茶树资源综合开发重点实验室开放基金(HNKLTOF2019006),中国农业科学院科技创新工程(CAAS-ASTIP-2022-TRICAAS)
作者单位
王伟伟 中国农业科学院茶叶研究所 
陈 义 河南省豫南茶树资源综合开发重点实验室 
杨丽娟 中国农业科学院茶叶研究所;中国农业科学院研究生院 
江和源 中国农业科学院茶叶研究所 
AuthorInstitution
WANG Wei-Wei Tea Research Institute, Chinese Academy of Agricultural Sciences 
CHEN Yi Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan 
YANG Li-Juan Tea Research Institute, Chinese Academy of Agricultural Sciences;Graduate School of Chinese Academy of Agricultural Sciences 
JIANG He-Yuan Tea Research Institute, Chinese Academy of Agricultural Sciences 
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中文摘要:
      目的 探明两种加工方式对信阳毛尖的化学成分影响。方法 以信阳群体种一芽一叶为原料, 分别按照手工和机制方式加工信阳毛尖, 采用超高效液相色谱-四极杆-静电轨道阱质谱法对非挥发性化合物进行分析。结果 本研究共鉴定出93个化合物, 包括生物碱类、氨基酸类、儿茶素类、儿茶素二聚物类、黄酮(醇)糖苷类、酚酸类、核苷类等。采用主成分分析(principal component analysis, PCA)和正交偏最小二乘法判别分析(orthogonal partial least squares-discriminant analysis, OPLS-DA)等方法, 对手工和机制加工信阳毛尖过程样的研究表明: 手工和机制加工过程样可以通过化学成分的组成进行较好地区分, 进一步分析两种加工方式发现了40种差异化合物, 其中黄酮(醇)糖苷类和氨基酸是差异最明显的两大类成分, 机制茶加工过程样中多数黄酮(醇)糖苷类和氨基酸含量高于手工制作, 手工茶的L-丙氨酸、γ-氨基丁酸、山奈酚3-O-半乳糖苷和槲皮素含量高于机制茶。手工茶制作过程中, 杀青温度较低、时间相对较长, 从而产生了更多的表阿夫儿茶精-3-没食子酸酯、原花青素B1、聚酯型儿茶素A和茶黄素双没食子酸酯等二聚物。此外, 手工茶的多数酚酸类化合物, 以及一磷酸腺苷和磷酸甘油胆碱等含量高于机制茶。结论 本研究采用代谢组学方法较为系统地分析了手工和机制信阳毛尖加工过程中的差异化合物, 为信阳毛尖手工茶和机制茶品质差异评价及加工技术提升提供了理论依据。
英文摘要:
      Objective To investigate the impact of 2 kinds of processing methods on the chemical composition of Xinyang Maojian. Methods Xinyang Maojian was processed hand-made and machine-made methods using one bud and one leaf of Xinyang population planting, an ultra performance liquid chromatography-quadrupole electrostatic orbitrap mass spectrometry was applied to analyze the non-volatile chemical components. Results A total of 93 compounds were structurally identified in this study, including alkaloids, amino acids, catechins, dimeric catechins, flavonoid (alcohol) glycosides, phenolic acids, nucleosides, etc.. The study between the hand-made and machine-made Xinyang Maojian processing was analysised by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), hand-made and machine-made processing samples could be well distinguished by the chemical components, it got 40 differential compounds after further analysis of two sets of process samples, among them, flavonoid (alcohol) glycosides and amino acids were the most differential components, the components of most flavonoids (alcohols) glycosides and amino acids in the processed samples of machine-made samples were higher than that in hand-made samples, L-alanine, γ-aminobutyric acid, kaempferol 3-O-galactoside, and quercetin contents in hand-made samples were higher than that in machine-made samples.Hand-made tea had more dimers catechins such as epiafzelechin 3-gallate, procyanidin B1, theasininsin A, and theaflavin digallate due to its low fixation temperature and long time. In addition, most phenolic acid compounds, as well as adenosine monophosphate and glycerophorine, in hand-made tea were higher than those in machine-made tea. Conclusion This study systematically analyze the differential compounds in the manual and mechanical processing of Xinyang Maojian used metabolomics methods, as well as provide theoretical basis for evaluating the quality differences and improving the processing technology between hand-made and machine-made Xinyang Maojian tea.
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