鱼胶品种的DNA条形码鉴定方法研究

Study of the molecular identification for isinglass on DNA

中文关键词:  鱼胶  DNA条形码  物种鉴定

英文关键词:isinglass  DNA barcodes  species identification

中文摘要:

      目的 建立鱼胶种属鉴别的DNA条形码方法。方法 使用不同试剂盒对鱼胶核酸提取效果进行评价和优化, 筛选出适用于鱼胶的核酸提取方法, 选取3对引物进行扩增对比, 建立鱼胶种属鉴别的DNA条形码方法。基于建立的方法, 对44份鱼胶样品进行特异性扩增, 并根据生命条形码系统(barcode of life data system, BOLD)进行物种鉴定, 同时进行系统进化分析。结果 对44份鱼胶样品的鉴定结果显示, 样品分属8科, 11属, 15种, 石首鱼科(15个)和尖吻鲈科(20个)占比79.5%。系统发育分析表明, 同物种的不同个体都聚在了一起, 同属以及同科不同属的物种也聚在一支, 界限分明。序列比对和系统发育分析较一致确认鱼胶所属的鱼类种类。结论 建立的方法能高效、准确地对鱼胶进行种属鉴定, 研究结果可有效预防和降低鱼胶产品交易中的欺诈行为, 也为促进我国鱼胶产品的高质量发展和进出口贸易的稳定发展提供有利的技术支持。

英文摘要:

      Objective To develop a method of DNA barcode for identification of isinglass. Methods The effect of different kits on nucleic acid extraction of isinglass was evaluated and optimized, and the nucleic acid extraction method suitable for isinglass was developed. Comparison and amplification 3 pairs of primers, and the DNA barcoding method for species identification of fish glue was established. Based on the established method, 44 isinglass samples were amplified specifically, species identification was performed according to barcode of life data system (BOLD) system, and phylogenetic analysis was performed. Results The results showed that the 44 isinglass samples belonged to 8 families, 11 genera and 15 species, and the proportion of species in the family Sciaenidae (15) and Latidae (20) was 79.5%. Phylogenetic analysis showed that different individuals of the same species were clustered together, and species of the same genus and different genera of the same family were clustered together in a well-defined line. Sequence comparison and phylogenetic analysis were consistent to identify the fish species of isinglass. Conclusion The established method can identify the species of isinglass efficiently and accurately. The research results can effectively prevent and reduce the fraudulent behavior in the trade of isinglass, and also provide favorable technical support for promoting the high-quality development of isinglass products and the stable development of import and export trade.